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Clone Read LE_HBa0061B22_SP6_47322

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0061B22_SP6_47322
Primer
SP6
ID from Seq. Facility
LE_HBa_061_B22-SP6
Chromatogram
Sequence 
Version
1 (current)
Length (bp)
27 trimmed for vector and quality, 879 raw
Sequence
>LE_HBa0061B22_SP6_47322 (raw untrimmed)
CCCGATGTGTNACANNANNNATNGTCNNNNNNNNNNANTATTNANTCATCTTTCTCNAGGCTTTGGTCCATCACCTTTAAAGAGGGGGNNANGANNNCNN
CNGCNNNCNNGGCCCCTTGGAGGGGNAAGNGAACNNGGNNNACAAAANGACAACNNNCACANANAGCAAANAAAAAANNCANACNNACACCGNNANAGCA
AAGNNNGCANAANNNGNGCNCCANAGCAAANANAAAACNGNANAANNNCGCNANGCANANACAANNNANANAANNNCGCNGGCCNANNNCGCNNGCAANN
GNANAANNNCGCNANCCNANNNGNANAANGCACAANNNANANAANNNCGCNGCGNANNNNCGCNGCAANANNNNGNANAAAANNNNANNNNNGCANACAA
NNNGAANNCGAAGNAAAANNGNANGNANANNNGCANAANNNANAAGNNGNANAGGAAAGAAGAAANANNGNNNNNCNNCNCGCNNNANACAAAAACANGA
AACACAANANANNACACNNCNGNNNGNANAAAGCNNAGAGAAAANNNGNANNNNCACNGCAANNNGNANAANNNCGCAANNNGNANAANNNCGNNGGCCN
NNNNNGCGCNGCAANANNNNGNANAAAANNNNGCANNNNANCNNACAANCAAANNNGAAGNAAAANNGANNGNAAANNNGNANNANNAAGNGNNANAGCA
CGAAGAAANANNANNNNNNGCANGNGAANANACAANNNNCNCNCGCNNNANACAAAANAGAAACNNCAANNNANACACNNCNGNGNAN
AAAGCGAGAGAG
GCGAACAGAGGGAGAGNGGCGANCGAGAANNNGGGAAGAGAGGCGACCGACAACNNNNGGCAACGNNNGCNAGGGAAAA
>LE_HBa0061B22_SP6_47322 (trimmed for quality and cloning vector)
AAAGCGAGAGAGGCGAACAGAGGGAGA

This is probably not a useful sequence.

Problems:
  • Low complexity
  • Short cloning insert
Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
1.96185
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0061B22
Clone
Clone name
hba-61b22
LE_HBa0061B22
C02HBa0061B22
P061B22
Clone type
BAC
Contamination
none
Estimated length
99,667
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found