Clone Read LE_HBa0001L04_T7_535

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001L04_T7_535
Primer
T7
ID from Seq. Facility
Le-HBa001_L04-T7.ab1
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
719 trimmed for vector and quality, 1258 raw
Genbank Accession
DU021014
Sequence
>LE_HBa0001L04_T7_535 (raw untrimmed)
ATACCCGGGCCCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCAATTACAACACATAAGGCGCAACCTTTCATTGTGAGGAATATTTTTC
TCATAACAATAAATCTCACCCTAGCACTTATGAGTAAATTATGCCAAAGTTCAACTTACCGACATGCGACTTGTCATTTCATAAACACTCACCATATAAA
AATGCATAATATGTCTCAAAACTGGCAAAACTTTTCAAGTAAAAATGCACAATTATTGTAATTCACTGTAACTACATAAAAAATGCGCCTTTTGCAAAAA
TTCACTAAAAATCATTGCAACTTAAACTTTAATCACTATTTTTATTATTAGTAAGAACTACTATCCTTAGTTATCAAATATATGAGGGCAACGGGAATTC
ATGTCGGCCTCGGCCTCCCATGTTGCACCCTCAACTAGGAGATTCTTCCATAACACATTTACGGAAGCTACTTATTTATTCCTCAATTTCTTTAATTGTC
TATCTAGTATTTGAACCAGAACTTTGTCCTAAAAAATGTTATCCTTCACACCAAGACTGTCAATGGGAACAATAGACTCGGGATCACTGATACACTTCTT
AAGCATGGAAACATGGAAAACCGGATGAACCGAAGCAAATGAACTAGACAACCTTACCAATGCTTTGCAATATTTCGTAGGAACATGCATAAAGAAGACT
CAACTTGCATTTTTTTCCATATCTAACCAGCCCTTTCATAGGCAAAATTTCCAAATACACCTTTTCACTGTAAGACCCAAATAAGAAGAATAGGCGTATA
GATCCTAACATATTCATATTAAGTTTATAAGGGCCTAGAAGGACTTATACTATTTTTTTGAACCAGTGTGAAAATTTCGAAGTGATTCGGAACTTAAACA
ATAAGGGACTCAAAAACTAAAAGGGACAACCAAAAGTTTCAACCACCTGTTTACCTATGGGGCGACAAAACCAATGCTTTTTTATAGACGGGATTCCTGA
CGTTAATATGTATTTAAATGGAATTAAACGCATTTTAATGGGGGGAAACAAAAATTAGAATAACTTAGGAGGGTCAACCAAATAAAAAAAGTTATAGCAT
TTGTAAAAAATACCCTTAAAAAATTTTAGGAAGTGTCTCAAGGGGGGCAAAGACGAATTCACCGGAATGAAAGGTTTTGTATGAGTATGGAGAAGCCATA
AGGGCGGGGTCAGGAGACGGCTGCGTTGTCTGGACTTTAGTTAGCGGGGACATCTTGT
>LE_HBa0001L04_T7_535 (trimmed for quality and cloning vector)
TAGAGTCGACCTGCAGGCATGCAAGCTTCAATTACAACACATAAGGCGCAACCTTTCATTGTGAGGAATATTTTTCTCATAACAATAAATCTCACCCTAG
CACTTATGAGTAAATTATGCCAAAGTTCAACTTACCGACATGCGACTTGTCATTTCATAAACACTCACCATATAAAAATGCATAATATGTCTCAAAACTG
GCAAAACTTTTCAAGTAAAAATGCACAATTATTGTAATTCACTGTAACTACATAAAAAATGCGCCTTTTGCAAAAATTCACTAAAAATCATTGCAACTTA
AACTTTAATCACTATTTTTATTATTAGTAAGAACTACTATCCTTAGTTATCAAATATATGAGGGCAACGGGAATTCATGTCGGCCTCGGCCTCCCATGTT
GCACCCTCAACTAGGAGATTCTTCCATAACACATTTACGGAAGCTACTTATTTATTCCTCAATTTCTTTAATTGTCTATCTAGTATTTGAACCAGAACTT
TGTCCTAAAAAATGTTATCCTTCACACCAAGACTGTCAATGGGAACAATAGACTCGGGATCACTGATACACTTCTTAAGCATGGAAACATGGAAAACCGG
ATGAACCGAAGCAAATGAACTAGACAACCTTACCAATGCTTTGCAATATTTCGTAGGAACATGCATAAAGAAGACTCAACTTGCATTTTTTTCCATATCT
AACCAGCCCTTTCATAGGC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.951183
Expected error %
0.556709
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38724.1| Score 121 E Value 3e-31 Identity 58.20% Align. Length 378bp (52.57%) Frame 2
putative retrotransposon protein [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38792.1| Score 114 E Value 1e-28 Identity 54.10% Align. Length 378bp (52.57%) Frame 2
putative gag-pol polyprotein [Solanum demissum] gb|AAT38791.1| putative gag-pol polyprotein [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001L04
Clone
Clone name
hba-1l4
LE_HBa0001L04
P001L04
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found