Clone Read LE_HBa0011D14_T7_5461

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0011D14_T7_5461
Primer
T7
ID from Seq. Facility
LE_HBa_011_D14-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
743 trimmed for vector and quality, 975 raw
Genbank Accession
DU025108
Sequence
>LE_HBa0011D14_T7_5461 (raw untrimmed)
CCAAAACCGGGCCCGAGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAAAAGATATTGCATTCAACAGCCTTTTCAGGCAAACATTCACAAGTG
GAATTAGATCAAGAAACCCTCTAGCTTCAAGTAGATACATTGTCTACAATACATAGGTAAATTCCTTTCTCTATAGTCTTGCATGTAGACATTATCAATT
TGTTCCTTTATCTAATGATCATCTTCATGGTTTCTGCAAATCAAAGATAGGAAGAACAATACTAATTCTTTTCTATACATGTGCATACTCATCATTTAGT
TTTTATTTCCTCTATCAACTTTCAGAAATTATAATCTGCACTAGTGTTGCAGGGTTCTTTTAGTTTTAGGAGATTGGAGTTGTAAAATAAAGTCAACTTT
TACTATTAGAGGAAGTGATGAAGAGTAATAATTATAACATATTAAGAAAATCACAAATATCATAACAACAGATTCCAAAAGTTACAAAAAGGATTCAAAA
TTCCTAATTAACTAGTATAAAATCTAAAACATAAAGGCTGACCTTAAAATCCAAGCTAGCTAAATTCATCGCAAAAAAAAAAAACTTAACATGTCATCAC
ATATTAGATCTTGCATTCCACATTGATTGAGCCAACTCATCCCTCCAAGTGGTCCACTCTTCAGACTATTCAACAAGATCAATATGTTCAGGTTGATTCT
CAACTTGTTCTTCCATATCCATGTCTAAAGGATCCACATCCATCTCTCTACGGATGTAGTTGTGAATCAAACAACAGCACTTATAATTCTGTTATGAACT
TTAACAGAGTACCACGAAAAACTCCTAAGAATTCCCCACGCCCTTTTCAAAAACCAAACGTCCTTTCGATAACATTACAAGCCCTGGCAGGCTTCTATTA
AAAAGTTATTCTCGAATTCAGGTTCGGGGTTGTCCCCTGCCAACCCTTAACCAAAACAAATCCCCGGTAAGGGAA
>LE_HBa0011D14_T7_5461 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTAAAAGATATTGCATTCAACAGCCTTTTCAGGCAAACATTCACAAGTGGAATTAGATCAAGAAACCCTCT
AGCTTCAAGTAGATACATTGTCTACAATACATAGGTAAATTCCTTTCTCTATAGTCTTGCATGTAGACATTATCAATTTGTTCCTTTATCTAATGATCAT
CTTCATGGTTTCTGCAAATCAAAGATAGGAAGAACAATACTAATTCTTTTCTATACATGTGCATACTCATCATTTAGTTTTTATTTCCTCTATCAACTTT
CAGAAATTATAATCTGCACTAGTGTTGCAGGGTTCTTTTAGTTTTAGGAGATTGGAGTTGTAAAATAAAGTCAACTTTTACTATTAGAGGAAGTGATGAA
GAGTAATAATTATAACATATTAAGAAAATCACAAATATCATAACAACAGATTCCAAAAGTTACAAAAAGGATTCAAAATTCCTAATTAACTAGTATAAAA
TCTAAAACATAAAGGCTGACCTTAAAATCCAAGCTAGCTAAATTCATCGCAAAAAAAAAAAACTTAACATGTCATCACATATTAGATCTTGCATTCCACA
TTGATTGAGCCAACTCATCCCTCCAAGTGGTCCACTCTTCAGACTATTCAACAAGATCAATATGTTCAGGTTGATTCTCAACTTGTTCTTCCATATCCAT
GTCTAAAGGATCCACATCCATCTCTCTACGGATGTAGTTGTGA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.94074
Expected error %
0.510887
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.17%) Frame 2
pT7T3D cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.17%) Frame 2
pT7T318U cloning vector
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0011D14
Clone
Clone name
hba-11d14
LE_HBa0011D14
C12HBa0011D14
P011D14
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found