Clone Read LE_HBa0147P20_T7_73335

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0147P20_T7_73335
Primer
T7
ID from Seq. Facility
LE_HBa_147_P20-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
600 trimmed for vector and quality, 862 raw
Genbank Accession
DU065794
Sequence
>LE_HBa0147P20_T7_73335 (raw untrimmed)
TGCCTGCTGGGACCGTGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGTTGCTCATTGGTAATGGTTCCGGTTCATATAGCAGAAAAGGAGGCC
GCCTACACCGTTGGATGAGGAGGAAGTAGCATTTAGAAGTAGTGTGACGCAGCGTTTGTAGATCTGATGACCTATGTGGGTGTCTTATCAAGCATTCTTG
GCGAGTTGCAAAAATATTTAGTTGGAACAGGATCAGTCTTTTCCTTTTAATCGGAGAAGATCACTAGTCCTTTGTCGAAAAATTGTACTGTCTGACCGCA
TTTGATTGATCATAAGGGAAAAATTAATGAAATGAAGTGCTACTATAGCAAGAGCTTGGGGTACTTGATATTAAAATTGATGAAGTAGCAACTGAAATCT
GTATTGTATTAGTTATACAGTTCCTTGAAATTTGCACTGTATCAATGTCAGTAATTCAATTTGTGTTATGAGATACACTTGTAAGAACTATCACCCTAAG
TTCATGTCTTGATTTTCTAGATACAACTCAAGAACAACCTAGCTAACTTTGAAAGATTAACCCCATAAACATTTCCAAGTTGAAGGGATTATGGAGATTA
GGTTAGTGTAGTGGATGCTCTTAGCTCTTACCTTAGAACCTCCTCTGGGATAATGTGTATTTCTTCAAATTGTGATTGTTGATTGGTAATCTATCCAATG
TTTTGATCCAACCATAAATTTATTGAATTAAATCCCTCTCTTCTCCCTCCTCTCTATTATTGTGGGTTTAATTGANCCCAAAAAGGGGCGGTTTTGGCCC
TTAGGGGCACCGGCTCCCGCTAAGGTTTACATTCCCTATAAAAAATTTACATTTAAAACGGG
>LE_HBa0147P20_T7_73335 (trimmed for quality and cloning vector)
TCTAGAGTCGACCTGCAGGCATGCAAGCTTGTTGCTCATTGGTAATGGTTCCGGTTCATATAGCAGAAAAGGAGGCCGCCTACACCGTTGGATGAGGAGG
AAGTAGCATTTAGAAGTAGTGTGACGCAGCGTTTGTAGATCTGATGACCTATGTGGGTGTCTTATCAAGCATTCTTGGCGAGTTGCAAAAATATTTAGTT
GGAACAGGATCAGTCTTTTCCTTTTAATCGGAGAAGATCACTAGTCCTTTGTCGAAAAATTGTACTGTCTGACCGCATTTGATTGATCATAAGGGAAAAA
TTAATGAAATGAAGTGCTACTATAGCAAGAGCTTGGGGTACTTGATATTAAAATTGATGAAGTAGCAACTGAAATCTGTATTGTATTAGTTATACAGTTC
CTTGAAATTTGCACTGTATCAATGTCAGTAATTCAATTTGTGTTATGAGATACACTTGTAAGAACTATCACCCTAAGTTCATGTCTTGATTTTCTAGATA
CAACTCAAGAACAACCTAGCTAACTTTGAAAGATTAACCCCATAAACATTTCCAAGTTGAAGGGATTATGGAGATTAGGTTAGTGTAGTGGATGCTCTTA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.967756
Expected error %
0.293781
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|Y10544.1:4398-4492 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (5.17%) Frame 2
Cloning vector pKIL194T
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|Y14438.1:4678-4775 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (5.17%) Frame 2
Escherichia coli cloning vector plasmid DNA pBHRK18
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0147P20
Clone
Clone name
hba-147p20
LE_HBa0147P20
P147P20
Clone type
BAC
Contamination
none
Estimated length
118,777
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found