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SC Spread ID	Images			Distance from centromere (percentage of arm length)
843-03 1	Composite	Fluorescence	Phase	45.9%
843-15 1	Composite	Fluorescence	Phase	50.0%
843-24 1	Composite	Fluorescence	Phase	47.8%
843-25 1	Composite	Fluorescence	Phase	49.0%
843-26 1	Composite	Fluorescence	Phase	49.5%
843-31 1	Composite	Fluorescence	Phase	51.7%
843-32 1	Composite	Fluorescence	Phase	51.3%
843-33 1	Composite	Fluorescence	Phase	47.3%
Mean±sd				49.1%±2.0%

Given an estimated length of 5.1µm for chromosome 4P and the mean percentage above, this BAC is approximately 2.5µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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