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SC Spread ID	Images			Distance from centromere (percentage of arm length)
169-04 1	Composite	Fluorescence	Phase	13.8%
169-05 1	Composite	Fluorescence	Phase	16.2%
169-06 1	Composite	Fluorescence	Phase	14.7%
169-13 1	Composite	Fluorescence	Phase	16.7%
169-22 1	Composite	Fluorescence	Phase	13.9%
169-23 1	Composite	Fluorescence	Phase	15.0%
169-29 1	Composite	Fluorescence	Phase	14.0%
169-33 1	Composite	Fluorescence	Phase	15.7%
169-34 1	Composite	Fluorescence	Phase	17.1%
169-35 1	Composite	Fluorescence	Phase	15.6%
169-36 1	Composite	Fluorescence	Phase	16.4%
169-38 1	Composite	Fluorescence	Phase	15.3%
169-39 1	Composite	Fluorescence	Phase	14.1%
169-41 1	Composite	Fluorescence	Phase	15.4%
Mean±sd				15.3%±1.1%

Given an estimated length of 15.7µm for chromosome 3Q and the mean percentage above, this BAC is approximately 2.4µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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