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SC Spread ID	Images			Distance from centromere (percentage of arm length)
354-11 1	Composite	Fluorescence	Phase	82.5%
354-15 1	Composite	Fluorescence	Phase	75.6%
354-16 1	Composite	Fluorescence	Phase	83.9%
354-18 1	Composite	Fluorescence	Phase	82.9%
354-22 1	Composite	Fluorescence	Phase	80.0%
354-37 1	Composite	Fluorescence	Phase	82.0%
354-45 1	Composite	Fluroescence	Phase	80.1%
354-46 1	Composite	Fluorescence	Phase	78.9%
354-49 1	Composite	Fluorescence	Phase	83.6%
354-50 1	Composite	Fluorescence	Phase	75.8%
354-51 1	Composite	Fluorescence	Phase	80.8%
Mean±sd				80.5%±2.9%

Given an estimated length of 4.8µm for chromosome 3P and the mean percentage above, this BAC is approximately 3.9µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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