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SC Spread ID	Images			Distance from centromere (percentage of arm length)
686-08 1	Composite	Fluorescence	Phase	36.9%
686-10 1	Composite	Fluorescence	Phase	32.6%
686-11 1	Composite	Fluorescence	Phase	34.9%
686-13 1	Composite	Fluorescence	Phase	33.6%
686-18 1	Composite	Fluorescence	Phase	37.9%
686-22 1	Composite	Fluorescence	Phase	37.0%
686-26 1	Composite	Fluorescence	Phase	33.1%
686-35 1	Composite	Fluorescence	Phase	38.6%
686-41 1	Composite	Fluorescence	Phase	33.0%
686-45 1	Composite	Fluorescence	Phase	34.4%
686-46 1	Composite	Fluorescence	Phase	37.0%
Mean±sd				35.4%±2.2%

Given an estimated length of 7.3µm for chromosome 12Q and the mean percentage above, this BAC is approximately 2.6µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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