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SC Spread ID	Images			Distance from centromere (percentage of arm length)
133-05 1	Composite	Fluorescence	Phase	83.5%
133-18 1	Composite	Fluorescence	Phase	83.0%
133-20 1	Composite	Fluorescence	Phase	83.7%
133-21 1	Composite	Fluorescence	Phase	81.3%
133-23 1	Composite	Fluorescence	Phase	84.2%
133-24 1	Composite	Fluorescence	Phase	82.9%
133-26 1	Composite	Fluorescence	Phase	84.8%
133-27 1	Composite	Fluorescence	Phase	83.6%
133-28 1	Composite	Fluorescence	Phase	86.4%
133-29 1	Composite	Fluorescence	Phase	81.4%
133-32 1	Composite	Fluorescence	Phase	84.4%
133-33 1	Composite	Fluorescence	Phase	86.1%
133-39 1	Composite	Fluorescence	Phase	84.4%
Mean±sd				83.8%±1.5%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 16.8µm±0.3µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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