SGN Marker SSR605

SGN-M1278

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 45 High: 45
Repeat motif: CAA   Repeat number: 6
Repeat motif:    Repeat number:
Forward primer: TGGCCGGCTTCTAGAAATAA
Reverse primer: TGAAATCACCCGTGACCTTT
Predicted size: 196
Derivations 
This marker was derived from EST read SGN-E121863
Mapped locations  
Map: Sun1642 x LA1589   
Map version
85 (location: 43815)
Chromosome
2
Position
1.90 cM
Confidence
uncalculated
Protocol
SSR


Sun1642 x LA1589

PCR data   Exp. ID 44831
Forward primer (5'-3')
TGGAAAGAAGCAGTAGCATTG
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
CAACGAACATCCTCCGTTCT
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589   
Map version
79 (location: 43275)
Chromosome
2
Position
50.30 cM
Confidence
uncalculated
Protocol
SSR


Integrated map: Yellow Stuffer X LA1589 and Sun1642 X LA1589

PCR data   Exp. ID 44645
Forward primer (5'-3')
TGGAAAGAAGCAGTAGCATTG
Accessions and product sizes
Approximate temperature
55°C
Reverse primer (5'-3')
CAACGAACATCCTCCGTTCT
Digested band sizes (using unknown enzyme)
Mg+2 concentration
Unknown
   Enzymes
  

Map: Tomato-EXPIMP 2008   
Map version
55 (location: 40807)
Chromosome
2
Position
44.00 cM
Confidence
uncalculated
Protocol
SSR


Tomato-EXPIMP 2008

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39279)
Chromosome
2
Position
48.50 cM
Confidence
I
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 27
Forward primer (5'-3')
TGGCCGGCTTCTAGAAATAA
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TGAAATCACCCGTGACCTTT
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 196+196
Solanum pennellii LA716: 176+196
Approximate temperature
55°C
  
Overgo hybridization and physical mapping 
SSR605 was used as an overgo probe on plate 12 [well D4]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
TACTTCCAACATCGCCGTCTCAGA
B sequence
ACCCGTGACCTTTTCTTCTGAGAC
AB sequence
TACTTCCAACATCGCCGTCTCAGAAGAAAA
Marker sequence
TTGAAGTATTTTACAAAAGCAAATATGGATGGAAGATTCAATAACTTTTT
TGTTTCTGAGCAAGAAGATTCCGAGAATTCACCGGAAAACAGCTCCGATT
CACCGCGTTCCGGTTTATTCAATGATAACAAGATGATCACTTCAACTTCA
TCTCCTAAAAGAAGCAGAAGATCCATCGAAAAAAGAGTGGTGTCAGTGCC
AATTAAAGAAATTGAAGGATCAAAAATGAAAGGTGAAATAAGTATGCCAC
CATCTGATTCTTGGGCATGGAGAAAATATGGACAAAAGCCCATCAAAGGT
TCCCCTTATCCCAGGGGATATTATAGATGTAGTAGTTCAAAAGGATGTCC
AGCTAGAAAACAAGTTGAAAGGAGCCGTGCGGACCCAAACATGTTAGTTG
TGACATATTCTTGTGAACATAACCATCCTTGGCCGGCTTCTAGAAATAAT
CAACACAACCATCGTACAACTACTAATACATCATGTATTATTAATAATAA
TAATAATACAAAGACGAAGATGAAAACAATAGTATCACTAACAGCAGCAG
CAACAACAACAACAACAACATCAACAACTACTTCCAACATCGCCGTCTCA
GAAGAAAAGGTCACGGGTGATTTCACACGTNCATCGGAGCCTAATTCGGA
CGAAAAATTTGTCAATCTCGGTGAATCATCATCGTCTT
Genomic location of SSR605  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D1278 marker 1278
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