Sol Genomics Network

SGN Marker cLET-8-K4

SGN-M2979

Synonyms

None

Locus associations

None

Derivations

This marker was derived from EST clone SGN-C88568

Mapped locations

Map: Eggplant-COSII
Map version 63 (location: 42506)
Chromosome 12
Position 35.90 cM
Confidence F(LOD3)
Protocol RFLP

Eggplant-COSII

Map: Tomato-EXPEN 2000
Map version 52 (location: 40424)
Chromosome 12
Position 41.00 cM
Confidence F(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

Map: Eggplant-LXM 2002
Map version 29 (location: 12295)
Chromosome 12
Position 37.00 cM
Confidence F(LOD3)
Protocol RFLP

Eggplant-LXM 2002

Other PCR data

PCR data Exp. ID 14067
Forward primer (5'-3') CACTTTGTGGCAATCGACAT	Digested band sizes (using DpnII) Solanum lycopersicum LA925: 1000 Solanum pennellii LA716: 900	Mg⁺² concentration Unknown
Reverse primer (5'-3') TGCCTTATGCCAAACAGAAA	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1100 Solanum lycopersicum LA925: 1100	Approximate temperature 55°C

PCR data Exp. ID 14067
Forward primer (5'-3') CACTTTGTGGCAATCGACAT	Digested band sizes (using DpnII) Solanum pennellii LA716: 900 Solanum lycopersicum LA925: 1000	Mg⁺² concentration Unknown
Reverse primer (5'-3') TGCCTTATGCCAAACAGAAA	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1100 Solanum pennellii LA716: 1100	Approximate temperature 55°C

PCR data Exp. ID 14154
Forward primer (5'-3') CACTTTGTGGCAATCGACAT	Digested band sizes (using TaqI) Solanum pimpinellifolium LA1589: 800+500 Solanum lycopersicum LA925: 500+350	Mg⁺² concentration Unknown
Reverse primer (5'-3') TGCCTTATGCCAAACAGAAA	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1200 Solanum lycopersicum LA925: 1200	Approximate temperature 55°C

PCR data Exp. ID 149
Forward primer (5'-3') Unknown	Digested band sizes (using DpnII) Solanum lycopersicum LA925: 1000 Solanum pennellii LA716: 900	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') Unknown	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1100 Solanum lycopersicum LA925: 1100	Approximate temperature 55°C

Overgo hybridization and physical mapping

cLET-8-K4 was used as an overgo probe on plate 4 [well D6]

Plausible BAC Matches: P180E20, P336A13, P196A15, P102I04, P215L09, P100H03, P248C03, P192D17, P217E12, P123B16, P104N14, P270M14, P159O09, P180O10, P189E07, P262D22, P292B18, P161B22, P116A07, P222K23, P161E19, P161C09

Overgo Sequences

A sequence

GGAAAGGATCTCGTTCTGTCTGTG

B sequence

CACCCATTGCAGACATCACAGACA

AB sequence

GGAAAGGATCTCGTTCTGTCTGTGATGTCT

Marker sequence

GCAAAATGTCACTTTGTGGCAATCGACATTTTCAATGCAAAAAAGCTTGA
AGATATTGTTCCTTCATCCCACAATTGTGATGTGCCACATGTCAATCGTA
CTGACTATCAGCTGATTGACATATCTGAAGATGGTTTTGTGTCTCTTCTT
ACTGAAAATGGAAACACCAAAGACGACCTCAGACTTCCCACCGATGACAC
CCTGTTGAACCAGGTTAAAGGTGGATTTGAGGAAGGAAAGGATCTCGTTC
TGTCTGTGATGTCTGCAATGGGTGAAGAGCAGATCTGTGCTGTGAAGGAC
ATTGGTACCAAGACCTAGTTGTGTGCATTCTGCAGCATAAATAATTGCTT
TTTAGCGAAGACGTTTTATATCTTGTTATCGTGGTACCTTTGCAATCTGT
TTTATCGTGAAAACACCTTATATCTATTGGCATGGCTTGAATAGTTGAAA
CTCTAATAGTTTCTGTTTGGCATAAGGCAATGAACTGGATTTGATAGCAG
AAATAATCTACATGTCACTTTTTTTTT

Genomic location of cLET-8-K4

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D2979 marker 2979