Sol Genomics Network

SGN Marker C2_At2g35920

SGN-M4636
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	311377 (SGN) 126871 (CGN)	-	-	-
Solanum tuberosum	Single	SGN-U256496	-	-	-
Arabidopsis	Single	At2g35920 on TAIR	-	-	-

Mapped locations

Map: Solanum michoacanum 2011
Map version 155 (location: 109230)
Chromosome 1
Position 13.50 cM
Confidence uncalculated
Protocol CAPS

Solanum michoacanum 2011

Map: Tomato-EXPEN 2000
Map version 52 (location: 38362)
Chromosome 1
Position 17.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39615

Forward primer (5'-3')

TGCTTGCAACCAACATAGCTGAG

Accessions and product sizes

Solanum pennellii TA56

400

Solanum lycopersicum TA209

400

Approximate temperature

55°C

Reverse primer (5'-3')

AAGCTCTTGTAGTGGTGTTCGAAG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

150+200

Solanum pennellii TA56

150+250

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25832)
Chromosome 2
Position 15.09 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10796
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 400	Approximate temperature 55°C

PCR data Exp. ID 10797
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 400	Approximate temperature 55°C

PCR data Exp. ID 10798
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 400	Approximate temperature 55°C

PCR data Exp. ID 13041
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 400	Approximate temperature 55°C

PCR data Exp. ID 13042
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 400	Approximate temperature 55°C

PCR data Exp. ID 13043
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 400	Approximate temperature 55°C

PCR data Exp. ID 13044
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 400	Approximate temperature 55°C

PCR data Exp. ID 13045
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 400	Approximate temperature 55°C

PCR data Exp. ID 13046
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 400	Approximate temperature 55°C

PCR data Exp. ID 13047
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Physalis TA1367: 400	Approximate temperature 55°C

PCR data Exp. ID 13048
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 400	Approximate temperature 55°C

PCR data Exp. ID 13049
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 400	Approximate temperature 55°C

PCR data Exp. ID 13050
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 400	Approximate temperature 55°C

PCR data Exp. ID 32951
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 400	Approximate temperature 55°C

PCR data Exp. ID 32952
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 400	Approximate temperature 55°C

PCR data Exp. ID 32953
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 400	Approximate temperature 55°C

PCR data Exp. ID 10799
Forward primer (5'-3') TGCTTGCAACCAACATAGCTGAG	Digested band sizes (using Tsp509I) Solanum lycopersicum LA925: 200+150 Solanum pennellii LA716: 250+150	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTTGTAGTGGTGTTCGAAG	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI,NheI
Accessions and product sizes Solanum lycopersicum LA925: 400 Solanum pennellii LA716: 400	Approximate temperature 55°C

Unigene blast matches for primers

SGN-U228754

Overgo hybridization and physical mapping

C2_At2g35920 was used as an overgo probe on plate 12 [well E11]

Plausible BAC Matches: P252O13, P126L02, P268K01, P119B03, P088A08

Overgo Sequences

A sequence

GGCGCTATTGAATCCTTTTTGGCC

B sequence

GAGGTTGAAGTGCCTTGGCCAAAA

AB sequence

GGCGCTATTGAATCCTTTTTGGCCAAGGCA

Marker sequence

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
AAAATTGTGCTTGCAACCAACATAGCTGAGAGCAGTATAACTATAGATGA
TGTCGTGTATGTCATAGACTGTGGGAAGGCGAAAGAGACTAGCTATGATG
CACTTAACAAGTTAGCTTGTTTATTACCTTCATGGATATCAAAGGCCTCA
GCACATCAGNNNNNAGGCGTGGTCGTGCGGGCCGTGTACAACCTGGAGTT
TGTTATAGGTTGTATCCGAAACTAATACATGATGCTATGGCACAATATCA
ACTACCTGAGATTCTTCGAACACCACTACAAGAGCTTTGTCTCCATATTA
AGAGTTTGCAGTTCGGCGCTATTGAATCCTTTTTGGCCAAGGCACTTCAA
CCTCCAGATGCTCTCTCTGTCCACAATGCTATTGAACTTCTCAAAACTAT
TGGAGCTCTGGATGATACAGAAGAACTTACTCCACTTGGCNNNNNCGCCA
TCTTTGCACTCTACCGCTGGATCCGAATATTGGAAAGATGCTTCTGATGG
GGTCCATCTTCCAATGTCTTAACCCTGCACTNNNNNNNNNNNNNNN

Universal primers for Asterid species

No additional PCR data found.

Other COSII sequence data

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Related Markers

Related Genotyped Markers: These markers have been genotyped and share a name with this marker

Genomic location of C2_At2g35920

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D4636 marker 4636

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Accessions	Seedlots
Accessions		Breeding Program	Seedlot Name	Contents	Seedlot Location	Count	Weight(g)

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