Sol Genomics Network

SGN-M4715
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301333 (SGN) 121452 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197505	-	-	-
Lycopersicon Combined	Single	SGN-U219076	-	-	-
Solanum tuberosum	Multiple	SGN-U246522	-	-	-
Arabidopsis	Single	At2g34470 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39610

Forward primer (5'-3')

TTGAGGGAAAAATACAGTCTTGC

Accessions and product sizes

Solanum lycopersicum TA209

630

Solanum pennellii TA56

630

Approximate temperature

55°C

Reverse primer (5'-3')

AAGAACTCTCCATCTTCTTTCGTG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

500

Solanum pennellii TA56

630

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10773
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 630	Approximate temperature 55°C

PCR data Exp. ID 10774
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 630	Approximate temperature 55°C

PCR data Exp. ID 10775
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 630	Approximate temperature 55°C

PCR data Exp. ID 13014
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 600	Approximate temperature 55°C

PCR data Exp. ID 13015
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 650	Approximate temperature 55°C

PCR data Exp. ID 13016
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 650	Approximate temperature 55°C

PCR data Exp. ID 13017
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650	Approximate temperature 55°C

PCR data Exp. ID 13018
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Physalis TA1367: 600	Approximate temperature 55°C

PCR data Exp. ID 32621
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 650	Approximate temperature 55°C

PCR data Exp. ID 32938
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 650	Approximate temperature 55°C

PCR data Exp. ID 32939
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 650	Approximate temperature 55°C

PCR data Exp. ID 32940
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 650	Approximate temperature 55°C

PCR data Exp. ID 32941
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650	Approximate temperature 55°C

PCR data Exp. ID 10776
Forward primer (5'-3') TTGAGGGAAAAATACAGTCTTGC	Digested band sizes (using MspI) Solanum pennellii LA716: 630 Solanum lycopersicum LA925: 500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGAACTCTCCATCTTCTTTCGTG	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI, RsaI
Accessions and product sizes Solanum pennellii LA716: 630 Solanum lycopersicum LA925: 630	Approximate temperature 55°C