Sol Genomics Network

SGN Marker C2_At2g24270

SGN-M4716
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	299764 (SGN) 121712 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U196761	-	-	-
Lycopersicon Combined	Single	SGN-U214796	-	-	-
Solanum tuberosum	Multiple	SGN-U244559	-	-	-
Arabidopsis	Single	At2g24270 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. acuminata
Map version 89 (location: 46461)
Chromosome 11
Position 3.10 cM
Confidence I(LOD2)
Protocol CAPS

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41374)
Chromosome 7
Position 21.40 cM
Confidence F(LOD3)
Protocol PCR

Pepper-COSII

PCR data Exp. ID 43350

Forward primer (5'-3')

TGGAAATGAAAGGACCAAATACTG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

AGGAGCAATCTTGGAGACGGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38321)
Chromosome 7
Position 0.30 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39581

Forward primer (5'-3')

TGGAAATGAAAGGACCAAATACTG

Accessions and product sizes

Solanum pennellii TA56

1000

Solanum lycopersicum TA209

1000

Approximate temperature

55°C

Reverse primer (5'-3')

AGGAGCAATCTTGGAGACGGC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

550

Solanum pennellii TA56

700

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25698)
Chromosome 2
Position 10.34 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10645
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1000	Approximate temperature 55°C

PCR data Exp. ID 10646
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1000	Approximate temperature 55°C

PCR data Exp. ID 10647
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1000	Approximate temperature 55°C

PCR data Exp. ID 12981
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1000	Approximate temperature 55°C

PCR data Exp. ID 12982
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1000	Approximate temperature 55°C

PCR data Exp. ID 12983
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 600	Approximate temperature 55°C

PCR data Exp. ID 12984
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 600	Approximate temperature 55°C

PCR data Exp. ID 12985
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 600	Approximate temperature 55°C

PCR data Exp. ID 12986
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 600	Approximate temperature 55°C

PCR data Exp. ID 12987
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Physalis TA1367: 500	Approximate temperature 55°C

PCR data Exp. ID 32923
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 600	Approximate temperature 55°C

PCR data Exp. ID 32924
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 600	Approximate temperature 55°C

PCR data Exp. ID 32925
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 600	Approximate temperature 55°C

PCR data Exp. ID 10648
Forward primer (5'-3') TGGAAATGAAAGGACCAAATACTG	Digested band sizes (using HinfI) Solanum lycopersicum LA925: 550 Solanum pennellii LA716: 700	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGGAGCAATCTTGGAGACGGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, HinfI All possible enzymes for lycopersicum and pimpinellifolium: AluI, HinfI
Accessions and product sizes Solanum pennellii LA716: 1000 Solanum lycopersicum LA925: 1000	Approximate temperature 55°C