Sol Genomics Network

SGN Marker C2_At3g13235

SGN-M4724
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	310006 (SGN) 125975 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197821	-	-	-
Lycopersicon Combined	Multiple	SGN-U217446	-	-	-
Solanum tuberosum	Single	SGN-U250857	-	-	-
Arabidopsis	Single	At3g13235 on TAIR	-	-	-

Mapped locations

Map: Eggplant-RAD 2012
Map version 174 (location: 132186)
Chromosome 4
Position 11.21 cM
Confidence uncalculated
Protocol CAPS

Eggplant-RAD 2012

Map: Tomato - Kazusa and SolCAP markers mapped to genome
Map version 105 (location: 49243)
Chromosome 10
Position 0.69 MB
Confidence uncalculated
Protocol CAPS

Tomato - Kazusa and SolCAP markers mapped to genome

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46661)
Chromosome 8
Position 12.80 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Eggplant-COSII
Map version 63 (location: 42215)
Chromosome 4
Position 0.00 cM
Confidence F(LOD3)
Protocol dCAPS

Eggplant-COSII

PCR data Exp. ID 43781

Forward primer (5'-3')

ATTCTGCTGCTGTCAAATGCTTTCG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

AGCATATCAAGTCCAAAGAGAAATTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Pepper-COSII
Map version 58 (location: 41412)
Chromosome 10
Position 0.00 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43388

Forward primer (5'-3')

AGGTCGTATTCATGTTGCTCCAATC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

AGCATATCAAGTCCAAAGAGAAATTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38461)
Chromosome 10
Position 0.00 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39701

Forward primer (5'-3')

AGGTCGTATTCATGTTGCTCCAATC

Accessions and product sizes

Solanum lycopersicum TA209

750

Solanum pennellii TA56

750

Approximate temperature

55°C

Reverse primer (5'-3')

AGCATATCAAGTCCAAAGAGAAATTCC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

210

Solanum pennellii TA56

250

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 26154)
Chromosome 3
Position 4.27 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11038
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 750	Approximate temperature 55°C

PCR data Exp. ID 11039
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 750	Approximate temperature 55°C

PCR data Exp. ID 11040
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 750	Approximate temperature 55°C

PCR data Exp. ID 13191
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 800	Approximate temperature 55°C

PCR data Exp. ID 13192
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 800	Approximate temperature 55°C

PCR data Exp. ID 13193
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 800	Approximate temperature 55°C

PCR data Exp. ID 13194
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 800	Approximate temperature 55°C

PCR data Exp. ID 13195
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 800	Approximate temperature 55°C

PCR data Exp. ID 13196
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 800	Approximate temperature 55°C

PCR data Exp. ID 13197
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Physalis TA1367: 800	Approximate temperature 55°C

PCR data Exp. ID 33014
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 800	Approximate temperature 55°C

PCR data Exp. ID 33015
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 800	Approximate temperature 55°C

PCR data Exp. ID 33016
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 800	Approximate temperature 55°C

PCR data Exp. ID 11041
Forward primer (5'-3') AGGTCGTATTCATGTTGCTCCAATC	Digested band sizes (using AluI) Solanum lycopersicum LA925: 210 Solanum pennellii LA716: 250	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCATATCAAGTCCAAAGAGAAATTCC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, RsaI
Accessions and product sizes Solanum lycopersicum LA925: 750 Solanum pennellii LA716: 750	Approximate temperature 55°C