Sol Genomics Network

SGN Marker C2_At5g16710

SGN-M4725
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301435 (SGN) 119940 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U214041	-	-	-
Solanum tuberosum	Single	SGN-U246755	-	-	-
Arabidopsis	Single	At5g16710 on TAIR	-	-	-

Mapped locations

Map: Eggplant-RAD 2012
Map version 174 (location: 132192)
Chromosome 11
Position 45.66 cM
Confidence uncalculated
Protocol CAPS

Eggplant-RAD 2012

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46756)
Chromosome 5
Position 84.90 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Tobacco N. acuminata
Map version 89 (location: 46527)
Chromosome 5
Position 75.00 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. acuminata

Map: Pepper Capsicum
Map version 65 (location: 42689)
Chromosome 12
Position 0.00 cM
Confidence uncalculated
Protocol PCR

Pepper Capsicum

Map: Pepper-COSII
Map version 58 (location: 41517)
Chromosome 12
Position 12.70 cM
Confidence I(LOD2)
Protocol PCR

Pepper-COSII

PCR data Exp. ID 43493

Forward primer (5'-3')

TGATGAGCTGACAGCTTTCAATGAT

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

AGTGAATCTGGAATAGACCAATTCTTAT

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38797)
Chromosome 11
Position 31.40 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39935

Forward primer (5'-3')

ACTTGATGAGCTGACAGCTTTCAATG

Accessions and product sizes

Solanum pennellii TA56

1400

Solanum lycopersicum TA209

1400

Approximate temperature

55°C

Reverse primer (5'-3')

AGCTTTGGTCCAAGCGACAAATC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

650

Solanum lycopersicum TA209

500

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27184)
Chromosome 5
Position 5.48 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11747
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1400	Approximate temperature 55°C

PCR data Exp. ID 11748
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1400	Approximate temperature 55°C

PCR data Exp. ID 11749
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1400	Approximate temperature 55°C

PCR data Exp. ID 13731
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1100	Approximate temperature 55°C

PCR data Exp. ID 13732
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 450	Approximate temperature 55°C

PCR data Exp. ID 13733
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 850	Approximate temperature 55°C

PCR data Exp. ID 13734
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 13735
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 100	Approximate temperature 55°C

PCR data Exp. ID 13736
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1100	Approximate temperature 55°C

PCR data Exp. ID 13737
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1150	Approximate temperature 55°C

PCR data Exp. ID 13738
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1300	Approximate temperature 55°C

PCR data Exp. ID 13739
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Physalis TA1367: 700	Approximate temperature 55°C

PCR data Exp. ID 13740
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 1300	Approximate temperature 55°C

PCR data Exp. ID 13741
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: Multiple	Approximate temperature 55°C

PCR data Exp. ID 13742
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1300	Approximate temperature 55°C

PCR data Exp. ID 13743
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 1300	Approximate temperature 55°C

PCR data Exp. ID 13744
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Coffea canephora BP409: 350	Approximate temperature 55°C

PCR data Exp. ID 33213
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 33214
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 100	Approximate temperature 55°C

PCR data Exp. ID 33215
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1100	Approximate temperature 55°C

PCR data Exp. ID 33216
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1150	Approximate temperature 55°C

PCR data Exp. ID 11750
Forward primer (5'-3') ACTTGATGAGCTGACAGCTTTCAATG	Digested band sizes (using AluI) Solanum lycopersicum LA925: 500 Solanum pennellii LA716: 650	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTTTGGTCCAAGCGACAAATC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, HaeIII
Accessions and product sizes Solanum pennellii LA716: 1400 Solanum lycopersicum LA925: 1400	Approximate temperature 55°C