Sol Genomics Network

SGN-M6570
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U197900	-	-	-
Lycopersicon Combined	Single	SGN-U223854	-	-	-
Solanum tuberosum	Single	SGN-U264912	-	-	-
Arabidopsis	Single	At1g10030 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39379

Forward primer (5'-3')

AGCTGTTAGGATGGTGGTTAATGC

Accessions and product sizes

Solanum pennellii TA56

1650

Solanum lycopersicum TA209

1650

Approximate temperature

53°C

Reverse primer (5'-3')

ACTCTGTCAAGAAATGACCGAAGGC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

500+1150

Solanum pennellii TA56

400+1150

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 9984
Forward primer (5'-3') AGCTGTTAGGATGGTGGTTAATGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTGTCAAGAAATGACCGAAGGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1500	Approximate temperature 55°C

PCR data Exp. ID 9985
Forward primer (5'-3') AGCTGTTAGGATGGTGGTTAATGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTGTCAAGAAATGACCGAAGGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1500	Approximate temperature 55°C

PCR data Exp. ID 9986
Forward primer (5'-3') AGCTGTTAGGATGGTGGTTAATGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTGTCAAGAAATGACCGAAGGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1500	Approximate temperature 55°C

PCR data Exp. ID 9987
Forward primer (5'-3') AGCTGTTAGGATGGTGGTTAATGC	Digested band sizes (using DpnII) Solanum pennellii LA716: 1100 Solanum lycopersicum LA925: 1500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTGTCAAGAAATGACCGAAGGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AdeI/DraIII,AluI,ApaLI,BanII,BsiHKAI,BslI,BsoBI,BstUI,BstYI,BtgI,DpnII,HinfI,PflFI,PflMI,RsaI,SsPI,SwaI,TspRI All possible enzymes for lycopersicum and pimpinellifolium: ApoI,BstZ17I,DraI,EcoRV,SwaI
Accessions and product sizes Solanum pennellii LA716: 1500 Solanum lycopersicum LA925: 1500	Approximate temperature 55°C