Sol Genomics Network

SGN-M6583
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U197262	-	-	-
Lycopersicon Combined	Single	SGN-U217847	-	-	-
Solanum tuberosum	Single	SGN-U247131	-	-	-
Arabidopsis	Single	At1g11750 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39384

Forward primer (5'-3')

TCGCCGGAGCATCAAAATGG

Accessions and product sizes

Solanum lycopersicum TA209

1650

Solanum pennellii TA56

1650

Approximate temperature

55°C

Reverse primer (5'-3')

TGCTTGAAAATCCAATATCTGATGAATC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 9999
Forward primer (5'-3') TCGCCGGAGCATCAAAATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTGAAAATCCAATATCTGATGAATC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 10000
Forward primer (5'-3') TCGCCGGAGCATCAAAATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTGAAAATCCAATATCTGATGAATC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 10001
Forward primer (5'-3') TCGCCGGAGCATCAAAATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTGAAAATCCAATATCTGATGAATC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 32198
Forward primer (5'-3') TCGCCGGAGCATCAAAATGG	Digested band sizes (using BstBI) Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 300	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCTTGAAAATCCAATATCTGATGAATC	Enzymes All possible enzymes for pennellii and and lycopersicum: BstBI,TaqI
Accessions and product sizes Solanum lycopersicum LA925: 350 Solanum pennellii LA716: 350	Approximate temperature 55°C