Sol Genomics Network

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SGN-M6713
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304340 (SGN) 121500 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U202895	-	-	-
Solanum tuberosum	Single	SGN-U252260	-	-	-
Arabidopsis	Single	At1g26640 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41064

Forward primer (5'-3')

TCAGAAAGTTTTATTGTTGTTCATGG

Accessions and product sizes

Solanum pennellii TA56

300

Solanum lycopersicum TA209

300

Approximate temperature

55°C

Reverse primer (5'-3')

TGAAATTCTTGTGGCAACAAAACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10107
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 300	Approximate temperature 55°C

PCR data Exp. ID 10108
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 300	Approximate temperature 55°C

PCR data Exp. ID 10109
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 300	Approximate temperature 55°C

PCR data Exp. ID 12536
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 300	Approximate temperature 55°C

PCR data Exp. ID 12537
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 300	Approximate temperature 55°C

PCR data Exp. ID 12538
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12539
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 300	Approximate temperature 55°C

PCR data Exp. ID 12540
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 300	Approximate temperature 55°C

PCR data Exp. ID 12541
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 300	Approximate temperature 55°C

PCR data Exp. ID 12542
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 300	Approximate temperature 55°C

PCR data Exp. ID 12543
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Physalis TA1367: 300	Approximate temperature 55°C

PCR data Exp. ID 12544
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 250	Approximate temperature 55°C

PCR data Exp. ID 12545
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 250	Approximate temperature 55°C

PCR data Exp. ID 32737
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 300	Approximate temperature 55°C

PCR data Exp. ID 32738
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 300	Approximate temperature 55°C

PCR data Exp. ID 32739
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 300	Approximate temperature 55°C

PCR data Exp. ID 32740
Forward primer (5'-3') TCAGAAAGTTTTATTGTTGTTCATGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAATTCTTGTGGCAACAAAACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 300	Approximate temperature 55°C