Sol Genomics Network

SGN Marker C2_At1g29320

SGN-M6742
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	309048 (SGN) 128031 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U218547	-	-	-
Solanum tuberosum	Single	SGN-U253696	-	-	-
Arabidopsis	Single	At1g29320 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. acuminata
Map version 89 (location: 46436)
Chromosome 3
Position 19.10 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. acuminata

Map: Eggplant-COSII
Map version 63 (location: 42179)
Chromosome 8
Position 0.00 cM
Confidence F(LOD3)
Protocol CAPS

Eggplant-COSII

PCR data Exp. ID 43743

Forward primer (5'-3')

ACTTTCTTATGCAAAGATGATCACCG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

GATAGGCTGGTCAACGTCATTTCA

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38154)
Chromosome 8
Position 0.00 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39437

Forward primer (5'-3')

ACTTTCTTATGCAAAGATGATCACCG

Accessions and product sizes

Solanum pennellii TA56

1650

Solanum lycopersicum TA209

1650

Approximate temperature

53°C

Reverse primer (5'-3')

TCTCTGAGCAGAAATATCATAAAGACG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25147)
Chromosome 1
Position 10.26 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10134
Forward primer (5'-3') ACTTTCTTATGCAAAGATGATCACCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGAGCAGAAATATCATAAAGACG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1200	Approximate temperature 55°C

PCR data Exp. ID 10135
Forward primer (5'-3') ACTTTCTTATGCAAAGATGATCACCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGAGCAGAAATATCATAAAGACG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1200	Approximate temperature 55°C

PCR data Exp. ID 10136
Forward primer (5'-3') ACTTTCTTATGCAAAGATGATCACCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGAGCAGAAATATCATAAAGACG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1200	Approximate temperature 55°C

PCR data Exp. ID 32209
Forward primer (5'-3') ACTTTCTTATGCAAAGATGATCACCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTCTGAGCAGAAATATCATAAAGACG	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI,AvaII,BstEII,BstNI,PflFI,PvuII,Sau96I,ScrFI,XmnI All possible enzymes for lycopersicum and pimpinellifolium: BstNI,ScrFI
Accessions and product sizes Solanum pennellii LA716: 1200 Solanum lycopersicum LA925: 1200	Approximate temperature 55°C