Sol Genomics Network

SGN-M6772
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U199935	-	-	-
Lycopersicon Combined	Single	SGN-U220897	-	-	-
Solanum tuberosum	Single	SGN-U257017	-	-	-
Arabidopsis	Single	At1g32070 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39449

Forward primer (5'-3')

TTGCATTCTAGGAAATTCTCATCAGG

Accessions and product sizes

Solanum lycopersicum TA209

Solanum pennellii TA56

Approximate temperature

55°C

Reverse primer (5'-3')

TTGCACGGGCCATGCCTATC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10168
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 250	Approximate temperature 55°C

PCR data Exp. ID 10169
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 250	Approximate temperature 55°C

PCR data Exp. ID 10170
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 250	Approximate temperature 55°C

PCR data Exp. ID 12585
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 200	Approximate temperature 55°C

PCR data Exp. ID 12586
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 200	Approximate temperature 55°C

PCR data Exp. ID 12587
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 200	Approximate temperature 55°C

PCR data Exp. ID 12588
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 200	Approximate temperature 55°C

PCR data Exp. ID 12589
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 200	Approximate temperature 55°C

PCR data Exp. ID 12590
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 200	Approximate temperature 55°C

PCR data Exp. ID 12591
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 200	Approximate temperature 55°C

PCR data Exp. ID 12592
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Physalis TA1367: 200	Approximate temperature 55°C

PCR data Exp. ID 12593
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 250	Approximate temperature 55°C

PCR data Exp. ID 12594
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 200	Approximate temperature 55°C

PCR data Exp. ID 12595
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 200	Approximate temperature 55°C

PCR data Exp. ID 12596
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 250	Approximate temperature 55°C

PCR data Exp. ID 32754
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 200	Approximate temperature 55°C

PCR data Exp. ID 32755
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 200	Approximate temperature 55°C

PCR data Exp. ID 32756
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 200	Approximate temperature 55°C

PCR data Exp. ID 32757
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 200	Approximate temperature 55°C

PCR data Exp. ID 10171
Forward primer (5'-3') TTGCATTCTAGGAAATTCTCATCAGG	Digested band sizes (using DpnII) Solanum lycopersicum LA925: 200 Solanum pennellii LA716: 250	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCACGGGCCATGCCTATC	Enzymes All possible enzymes for pennellii and and lycopersicum: DpnII
Accessions and product sizes Solanum lycopersicum LA925: 250 Solanum pennellii LA716: 250	Approximate temperature 55°C