Sol Genomics Network

SGN-M6793
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	299414 (SGN) 123368 (CGN)	-	-	-
Solanum tuberosum	Single	SGN-U243390	-	-	-
Arabidopsis	Single	At1g35720 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39455

Forward primer (5'-3')

ATTCTGGCTCATAGGAATGCAGCAC

Accessions and product sizes

Solanum lycopersicum TA209

250

Solanum pennellii TA56

250

Approximate temperature

53°C

Reverse primer (5'-3')

ATAAGAGGAACCAAAAGCTTACGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10180
Forward primer (5'-3') ATTCTGGCTCATAGGAATGCAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATAAGAGGAACCAAAAGCTTACGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 10181
Forward primer (5'-3') ATTCTGGCTCATAGGAATGCAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATAAGAGGAACCAAAAGCTTACGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 10182
Forward primer (5'-3') ATTCTGGCTCATAGGAATGCAGCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATAAGAGGAACCAAAAGCTTACGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 12184
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 12185
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1200	Approximate temperature 55°C

PCR data Exp. ID 12186
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12187
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1300	Approximate temperature 55°C

PCR data Exp. ID 12188
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1300	Approximate temperature 55°C

PCR data Exp. ID 12189
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Physalis TA1367: 1300+220	Approximate temperature 55°C

PCR data Exp. ID 12190
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 1300	Approximate temperature 55°C

PCR data Exp. ID 12191
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1300	Approximate temperature 55°C

PCR data Exp. ID 12192
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Coffea canephora BP409: 1300+400	Approximate temperature 55°C

PCR data Exp. ID 33314
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: Multiple	Approximate temperature 55°C

PCR data Exp. ID 33315
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1300	Approximate temperature 55°C

PCR data Exp. ID 33316
Forward primer (5'-3') TCTGGACTTTGAGTCCTGCTGAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTGTCTCCAGCAATTGCACGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1300	Approximate temperature 55°C

PCR data Exp. ID 10183
Forward primer (5'-3') ATTCTGGCTCATAGGAATGCAGCAC	Digested band sizes (using TaqI) Solanum lycopersicum LA925: 500+350 Solanum pennellii LA716: 850+500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATAAGAGGAACCAAAAGCTTACGG	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI, TaqI
Accessions and product sizes Solanum lycopersicum LA925: 1350 Solanum pennellii LA716: 1350	Approximate temperature 55°C