Sol Genomics Network

SGN Marker C2_At1g53000

SGN-M6856
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	304055 (SGN) 124971 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201101	-	-	-
Lycopersicon Combined	Single	SGN-U216303	-	-	-
Solanum tuberosum	Single	SGN-U263773	-	-	-
Arabidopsis	Single	At1g53000 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38200)
Chromosome 10
Position 7.50 cM
Confidence F(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39478

Forward primer (5'-3')

AGATTCTCGGCAAGCCTATGATCC

Accessions and product sizes

Solanum pennellii TA56

350

Solanum lycopersicum TA209

350

Approximate temperature

55°C

Reverse primer (5'-3')

AAGCTTTGCCCTTTCCCATGTTC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

300

Solanum lycopersicum TA209

350

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25265)
Chromosome 1
Position 19.75 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10264
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1800	Approximate temperature 55°C

PCR data Exp. ID 10265
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1800	Approximate temperature 55°C

PCR data Exp. ID 10266
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1800	Approximate temperature 55°C

PCR data Exp. ID 12691
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12692
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Solanum melongena TA2986: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12693
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12694
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 12695
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1100+350	Approximate temperature 55°C

PCR data Exp. ID 12696
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 12697
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 12698
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1200	Approximate temperature 55°C

PCR data Exp. ID 12699
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Physalis TA1367: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12700
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 600+900	Approximate temperature 55°C

PCR data Exp. ID 12701
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 850+550	Approximate temperature 55°C

PCR data Exp. ID 12702
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 750+550	Approximate temperature 55°C

PCR data Exp. ID 12703
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 600+800	Approximate temperature 55°C

PCR data Exp. ID 12704
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Coffea canephora BP409: 350	Approximate temperature 55°C

PCR data Exp. ID 32794
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1200	Approximate temperature 55°C

PCR data Exp. ID 32795
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1100+350	Approximate temperature 55°C

PCR data Exp. ID 32796
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1200	Approximate temperature 55°C

PCR data Exp. ID 32797
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 10267
Forward primer (5'-3') AGATTCTCGGCAAGCCTATGATCC	Digested band sizes (using DpnII) Solanum lycopersicum LA925: 700+500 Solanum pennellii LA716: 650	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTTTGCCCTTTCCCATGTTC	Enzymes All possible enzymes for pennellii and and lycopersicum: TaqI,DpnII All possible enzymes for lycopersicum and pimpinellifolium: DpnII
Accessions and product sizes Solanum lycopersicum LA925: 1800 Solanum pennellii LA716: 1800	Approximate temperature 55°C