Sol Genomics Network

SGN Marker C2_At1g60640

SGN-M6913
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U200460	-	-	-
Lycopersicon Combined	Single	SGN-U225406	-	-	-
Solanum tuberosum	Single	SGN-U257149	-	-	-
Arabidopsis	Single	At1g60640 on TAIR	-	-	-

Mapped locations

Map: Eggplant-RAD 2012
Map version 174 (location: 132181)
Chromosome 2
Position 13.86 cM
Confidence uncalculated
Protocol CAPS

Eggplant-RAD 2012

Map: Tomato-EXPEN 2000
Map version 52 (location: 38219)
Chromosome 2
Position 12.50 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39491

Forward primer (5'-3')

TCTGACAGGGGGAAATGAATCTTC

Accessions and product sizes

Solanum lycopersicum TA209

750

Solanum pennellii TA56

850

Approximate temperature

55°C

Reverse primer (5'-3')

ACTTCATTGAAAGAGCTATCTTCACTCTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

750

Solanum pennellii TA56

850

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25324)
Chromosome 1
Position 22.34 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10315
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 530	Approximate temperature 55°C

PCR data Exp. ID 10317
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 530	Approximate temperature 55°C

PCR data Exp. ID 12744
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 500	Approximate temperature 55°C

PCR data Exp. ID 12745
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 550	Approximate temperature 55°C

PCR data Exp. ID 12746
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 550	Approximate temperature 55°C

PCR data Exp. ID 12747
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 650	Approximate temperature 55°C

PCR data Exp. ID 12748
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 650	Approximate temperature 55°C

PCR data Exp. ID 12749
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650+550	Approximate temperature 55°C

PCR data Exp. ID 12750
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 650+550	Approximate temperature 55°C

PCR data Exp. ID 12751
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Physalis TA1367: 550	Approximate temperature 55°C

PCR data Exp. ID 12752
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 650	Approximate temperature 55°C

PCR data Exp. ID 12753
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 650	Approximate temperature 55°C

PCR data Exp. ID 32220
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 530	Approximate temperature 55°C

PCR data Exp. ID 32813
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 650	Approximate temperature 55°C

PCR data Exp. ID 32814
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 650	Approximate temperature 55°C

PCR data Exp. ID 32815
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 650+550	Approximate temperature 55°C

PCR data Exp. ID 32816
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 650+550	Approximate temperature 55°C

PCR data Exp. ID 10318
Forward primer (5'-3') TCTGACAGGGGGAAATGAATCTTC	Digested band sizes (using EcoRV) Solanum pennellii LA716: 400 Solanum lycopersicum LA925: 530	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTTCATTGAAAGAGCTATCTTCACTCTC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI, DraI, EcoRV, DpnII All possible enzymes for lycopersicum and pimpinellifolium: TaqI
Accessions and product sizes Solanum pennellii LA716: 530 Solanum lycopersicum LA925: 530	Approximate temperature 55°C