Sol Genomics Network

SGN-M6950
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	311195 (SGN) 128069 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U196127	-	-	-
Solanum tuberosum	Single	SGN-U256503	-	-	-
Arabidopsis	Single	At1g64660 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41137

Forward primer (5'-3')

TCCATCGAGGCCTCCGCCAC

Accessions and product sizes

Solanum lycopersicum TA209

400

Solanum pennellii TA56

400

Approximate temperature

55°C

Reverse primer (5'-3')

AGCGTCTGCGAAGCCACCACG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10349
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCGTCTGCGAAGCCACCACG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 400	Approximate temperature 55°C

PCR data Exp. ID 10350
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCGTCTGCGAAGCCACCACG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 400	Approximate temperature 55°C

PCR data Exp. ID 10351
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCGTCTGCGAAGCCACCACG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 400	Approximate temperature 55°C

PCR data Exp. ID 12215
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 580	Approximate temperature 55°C

PCR data Exp. ID 12216
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 650+500	Approximate temperature 55°C

PCR data Exp. ID 12217
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 500+800	Approximate temperature 55°C

PCR data Exp. ID 12218
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 500	Approximate temperature 55°C

PCR data Exp. ID 12219
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500+600	Approximate temperature 55°C

PCR data Exp. ID 12220
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500+600	Approximate temperature 55°C

PCR data Exp. ID 12221
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 12222
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 500	Approximate temperature 55°C

PCR data Exp. ID 12223
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Physalis TA1367: 500+850	Approximate temperature 55°C

PCR data Exp. ID 12224
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 500	Approximate temperature 55°C

PCR data Exp. ID 12225
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 320+500	Approximate temperature 55°C

PCR data Exp. ID 12226
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Coffea canephora BP409: 500	Approximate temperature 55°C

PCR data Exp. ID 33325
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500+600	Approximate temperature 55°C

PCR data Exp. ID 33326
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500+600	Approximate temperature 55°C

PCR data Exp. ID 33327
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 500	Approximate temperature 55°C

PCR data Exp. ID 33328
Forward primer (5'-3') TCCATCGAGGCCTCCGCCAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACGGAGCAAAAGTGTTGTCCACCAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 500	Approximate temperature 55°C