Sol Genomics Network

SGN-M6993
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	309910 (SGN) 126940 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U217827	-	-	-
Solanum tuberosum	Single	SGN-U253755	-	-	-
Arabidopsis	Single	At1g68100 on TAIR	-	-	-

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46608)
Chromosome 4
Position 31.00 cM
Confidence F(LOD3)
Protocol dCAPS

Tobacco N. tomentosiformis

Tomato-EXPEN 2000

PCR data Exp. ID 39517

Forward primer (5'-3')

TTGACTGGAGAGGAACAGGCTAAATC

Accessions and product sizes

Solanum lycopersicum TA209

350

Solanum pennellii TA56

350

Approximate temperature

55°C

Reverse primer (5'-3')

AAGCCATCCCATCAGTAAAATTGTG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

350

Solanum lycopersicum TA209

300

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10386
Forward primer (5'-3') TTGACTGGAGAGGAACAGGCTAAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCCATCCCATCAGTAAAATTGTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 200	Approximate temperature 55°C

PCR data Exp. ID 10387
Forward primer (5'-3') TTGACTGGAGAGGAACAGGCTAAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCCATCCCATCAGTAAAATTGTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 200	Approximate temperature 55°C

PCR data Exp. ID 10388
Forward primer (5'-3') TTGACTGGAGAGGAACAGGCTAAATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCCATCCCATCAGTAAAATTGTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 200	Approximate temperature 55°C

PCR data Exp. ID 32224
Forward primer (5'-3') TTGACTGGAGAGGAACAGGCTAAATC	Digested band sizes (using RsaI) Solanum pennellii LA716: 200 Solanum lycopersicum LA925: 170	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCCATCCCATCAGTAAAATTGTG	Enzymes All possible enzymes for pennellii and and lycopersicum: RsaI
Accessions and product sizes Solanum lycopersicum LA925: 200 Solanum pennellii LA716: 200	Approximate temperature 55°C