Sol Genomics Network

SGN Marker C2_At1g69420

SGN-M7005
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302941 (SGN) 121730 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U221736	-	-	-
Arabidopsis	Single	At1g69420 on TAIR	-	-	-

Mapped locations

Map: Tomato - Kazusa and SolCAP markers mapped to genome
Map version 105 (location: 49222)
Chromosome 5
Position 5.96 MB
Confidence uncalculated
Protocol CAPS

Tomato - Kazusa and SolCAP markers mapped to genome

Map: Kazusa F2-2000 genetic map
Map version 103 (location: 47153)
Chromosome 5
Position 74.50 cM
Confidence uncalculated
Protocol unknown

Kazusa F2-2000 genetic map

Map: Eggplant-COSII
Map version 63 (location: 42193)
Chromosome 10
Position 69.80 cM
Confidence F(LOD3)
Protocol CAPS

Eggplant-COSII

PCR data Exp. ID 43757

Forward primer (5'-3')

TCATTGCTCTCAGAGATCAAGAGCAAC

Accessions and product sizes

Approximate temperature

50°C

Reverse primer (5'-3')

TGGTTTCAAAGGGTAACTCTGCAGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Pepper-COSII
Map version 58 (location: 41348)
Chromosome 11
Position 29.30 cM
Confidence I(LOD2)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43324

Forward primer (5'-3')

TCATTGCTCTCAGAGATCAAGAGCAAC

Accessions and product sizes

Approximate temperature

53°C

Reverse primer (5'-3')

TGGTTTCAAAGGGTAACTCTGCAGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38249)
Chromosome 5
Position 62.70 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39520

Forward primer (5'-3')

TCATTGCTCTCAGAGATCAAGAGCAAC

Accessions and product sizes

Solanum pennellii TA56

1350+700

Solanum lycopersicum TA209

1350

Approximate temperature

55°C

Reverse primer (5'-3')

TGGTTTCAAAGGGTAACTCTGCAGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25418)
Chromosome 1
Position 26.10 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10392
Forward primer (5'-3') TCATTGCTCTCAGAGATCAAGAGCAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTTTCAAAGGGTAACTCTGCAGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1300	Approximate temperature 55°C

PCR data Exp. ID 10393
Forward primer (5'-3') TCATTGCTCTCAGAGATCAAGAGCAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTTTCAAAGGGTAACTCTGCAGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1300	Approximate temperature 55°C

PCR data Exp. ID 10394
Forward primer (5'-3') TCATTGCTCTCAGAGATCAAGAGCAAC		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGGTTTCAAAGGGTAACTCTGCAGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1250	Approximate temperature 55°C

PCR data Exp. ID 32225
Forward primer (5'-3') TCATTGCTCTCAGAGATCAAGAGCAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTTTCAAAGGGTAACTCTGCAGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1250	Approximate temperature 55°C

PCR data Exp. ID 32226
Forward primer (5'-3') TCATTGCTCTCAGAGATCAAGAGCAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGGTTTCAAAGGGTAACTCTGCAGG	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI,BanI,BsaBI,BslI,BsoBI,DpnII,MspI,NgoMIV All possible enzymes for lycopersicum and pimpinellifolium: AluI,ApoI,SsPI
Accessions and product sizes Solanum lycopersicum LA925: 1300 Solanum pennellii LA716: 1300	Approximate temperature 55°C