Sol Genomics Network

SGN Marker C2_At1g74520

SGN-M7050
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301586 (SGN) 124279 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201989	-	-	-
Lycopersicon Combined	Single	SGN-U218741	-	-	-
Solanum tuberosum	Single	SGN-U254549	-	-	-
Arabidopsis	Single	At1g74520 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38260)
Chromosome 3
Position 113.00 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39531

Forward primer (5'-3')

ACCAAATCCCCTGTGGATGATCAG

Accessions and product sizes

Solanum pennellii TA56

1350

Solanum lycopersicum TA209

1350

Approximate temperature

55°C

Reverse primer (5'-3')

AAGTAAGGTATGACCAACCAGCAG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

390+410

Solanum pennellii TA56

1350

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25463)
Chromosome 1
Position 28.01 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10415
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 10416
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 10417
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 12798
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1300	Approximate temperature 55°C

PCR data Exp. ID 12799
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1300	Approximate temperature 55°C

PCR data Exp. ID 12800
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1300	Approximate temperature 55°C

PCR data Exp. ID 12801
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1500	Approximate temperature 55°C

PCR data Exp. ID 12802
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1300	Approximate temperature 55°C

PCR data Exp. ID 12803
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1300	Approximate temperature 55°C

PCR data Exp. ID 12804
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Physalis TA1367: 1300	Approximate temperature 55°C

PCR data Exp. ID 12805
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 1500	Approximate temperature 55°C

PCR data Exp. ID 12806
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 1200	Approximate temperature 55°C

PCR data Exp. ID 32607
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1200	Approximate temperature 55°C

PCR data Exp. ID 32842
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1200	Approximate temperature 55°C

PCR data Exp. ID 32843
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1200	Approximate temperature 55°C

PCR data Exp. ID 32844
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1500	Approximate temperature 55°C

PCR data Exp. ID 32845
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1300	Approximate temperature 55°C

PCR data Exp. ID 10418
Forward primer (5'-3') ACCAAATCCCCTGTGGATGATCAG	Digested band sizes (using RsaI) Solanum lycopersicum LA925: 410+390 Solanum pennellii LA716: 1350	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGTAAGGTATGACCAACCAGCAG	Enzymes All possible enzymes for pennellii and and lycopersicum: RsaI, DpnII
Accessions and product sizes Solanum pennellii LA716: 1350 Solanum lycopersicum LA925: 1350	Approximate temperature 55°C