Sol Genomics Network

SGN Marker C2_At1g75350

SGN-M7057
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U205003	-	-	-
Lycopersicon Combined	Single	SGN-U217851	-	-	-
Solanum tuberosum	Single	SGN-U252156	-	-	-
Arabidopsis	Single	At1g75350 on TAIR	-	-	-

Mapped locations

Map: Pepper-COSII
Map version 58 (location: 41353)
Chromosome 4
Position 140.60 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43329

Forward primer (5'-3')

AATGTCGCTCCTCCTTCATTCTCC

Accessions and product sizes

Approximate temperature

60°C

Reverse primer (5'-3')

ATGTATATCCTTCTTCCTGCAGCTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38263)
Chromosome 4
Position 120.50 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39533

Forward primer (5'-3')

AATGTCGCTCCTCCTTCATTCTCC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ATGTATATCCTTCTTCCTGCAGCTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25471)
Chromosome 1
Position 28.28 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10429
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 150	Approximate temperature 55°C

PCR data Exp. ID 10430
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 150	Approximate temperature 55°C

PCR data Exp. ID 10431
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 150	Approximate temperature 55°C

PCR data Exp. ID 12827
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 700	Approximate temperature 55°C

PCR data Exp. ID 12828
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 200	Approximate temperature 55°C

PCR data Exp. ID 12829
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 200	Approximate temperature 55°C

PCR data Exp. ID 12830
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 200	Approximate temperature 55°C

PCR data Exp. ID 12831
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 200	Approximate temperature 55°C

PCR data Exp. ID 32609
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 200	Approximate temperature 55°C

PCR data Exp. ID 32853
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 200	Approximate temperature 55°C

PCR data Exp. ID 32854
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 200	Approximate temperature 55°C

PCR data Exp. ID 32855
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 200	Approximate temperature 55°C

PCR data Exp. ID 10432
Forward primer (5'-3') AATGTCGCTCCTCCTTCATTCTCC	Digested band sizes (using DraI) Solanum lycopersicum LA925: 150 Solanum pennellii LA716: 100	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGTATATCCTTCTTCCTGCAGCTCC	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI, DraI, EcoRV, HaeIII
Accessions and product sizes Solanum pennellii LA716: 150 Solanum lycopersicum LA925: 150	Approximate temperature 55°C