Sol Genomics Network

SGN-M7084
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	310741 (SGN) 130636 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U204023	-	-	-
Lycopersicon Combined	Single	SGN-U226038	-	-	-
Solanum tuberosum	Single	SGN-U258319	-	-	-
Arabidopsis	Single	At1g77290 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41175

Forward primer (5'-3')

AAGAACATGGATGCATTTTTCTTC

Accessions and product sizes

Solanum lycopersicum TA209

450

Solanum pennellii TA56

2000

Approximate temperature

55°C

Reverse primer (5'-3')

AGCCATTCGGGCAATTATTACACG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10449
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 2000	Approximate temperature 55°C

PCR data Exp. ID 10450
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 450	Approximate temperature 55°C

PCR data Exp. ID 10451
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 450	Approximate temperature 55°C

PCR data Exp. ID 12844
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 150	Approximate temperature 55°C

PCR data Exp. ID 12845
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Solanum melongena TA2986: 500	Approximate temperature 55°C

PCR data Exp. ID 12846
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 600	Approximate temperature 55°C

PCR data Exp. ID 12847
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 450	Approximate temperature 55°C

PCR data Exp. ID 12848
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 450	Approximate temperature 55°C

PCR data Exp. ID 12849
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 400	Approximate temperature 55°C

PCR data Exp. ID 12850
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 400	Approximate temperature 55°C

PCR data Exp. ID 12851
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Physalis TA1367: 700	Approximate temperature 55°C

PCR data Exp. ID 12852
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 700	Approximate temperature 55°C

PCR data Exp. ID 12853
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 700	Approximate temperature 55°C

PCR data Exp. ID 12854
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 700	Approximate temperature 55°C

PCR data Exp. ID 12855
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 700	Approximate temperature 55°C

PCR data Exp. ID 12856
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Coffea canephora BP409: 650	Approximate temperature 55°C

PCR data Exp. ID 32863
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 450	Approximate temperature 55°C

PCR data Exp. ID 32864
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 450	Approximate temperature 55°C

PCR data Exp. ID 32865
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 400	Approximate temperature 55°C

PCR data Exp. ID 32866
Forward primer (5'-3') AAGAACATGGATGCATTTTTCTTC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCCATTCGGGCAATTATTACACG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 400	Approximate temperature 55°C