Sol Genomics Network

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SGN Marker C2_At1g77470

SGN-M7089
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302847 (SGN) 124480 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197603	-	-	-
Lycopersicon Combined	Single	SGN-U222849	-	-	-
Solanum tuberosum	Single	SGN-U250470	-	-	-
Arabidopsis	Single	At1g77470 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38268)
Chromosome 6
Position 39.10 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39538

Forward primer (5'-3')

TGCCCTACAATCACGATGTACACG

Accessions and product sizes

Solanum lycopersicum TA209

2500

Solanum pennellii TA56

2000

Approximate temperature

55°C

Reverse primer (5'-3')

AAACCACCCTCAGGGACATCAAG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

2000

Solanum lycopersicum TA209

2500

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25503)
Chromosome 1
Position 29.12 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10455
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 2000	Approximate temperature 55°C

PCR data Exp. ID 10456
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 2500	Approximate temperature 55°C

PCR data Exp. ID 10457
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2500	Approximate temperature 55°C

PCR data Exp. ID 12857
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1500	Approximate temperature 55°C

PCR data Exp. ID 12858
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1500	Approximate temperature 55°C

PCR data Exp. ID 12859
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 300	Approximate temperature 55°C

PCR data Exp. ID 12860
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 300	Approximate temperature 55°C

PCR data Exp. ID 12861
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 300	Approximate temperature 55°C

PCR data Exp. ID 12862
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Physalis TA1367: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12863
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 250	Approximate temperature 55°C

PCR data Exp. ID 12864
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 250	Approximate temperature 55°C

PCR data Exp. ID 12865
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 250	Approximate temperature 55°C

PCR data Exp. ID 32612
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 2000	Approximate temperature 55°C

PCR data Exp. ID 32867
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2000	Approximate temperature 55°C

PCR data Exp. ID 32868
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: Multiple	Approximate temperature 55°C

PCR data Exp. ID 32869
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 300	Approximate temperature 55°C

PCR data Exp. ID 32870
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 300	Approximate temperature 55°C

PCR data Exp. ID 10458
Forward primer (5'-3') TGCCCTACAATCACGATGTACACG	Digested band sizes (using amplicon difference) Solanum lycopersicum LA925: 2500 Solanum pennellii LA716: 2000	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAACCACCCTCAGGGACATCAAG	Enzymes All possible enzymes for pennellii and and lycopersicum: amplicon difference
Accessions and product sizes Solanum lycopersicum LA925: 2500 Solanum pennellii LA716: 2000	Approximate temperature 55°C