Sol Genomics Network

SGN-M7181
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300552 (SGN) 123428 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U198033	-	-	-
Lycopersicon Combined	Multiple	SGN-U214814	-	-	-
Solanum tuberosum	Single	SGN-U246679	-	-	-
Arabidopsis	Single	At2g06050 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41199

Forward primer (5'-3')

TGCCAGGGATTTTCACAAAGGAAC

Accessions and product sizes

Solanum pennellii TA56

100

Solanum lycopersicum TA209

100

Approximate temperature

55°C

Reverse primer (5'-3')

AGATGCACGACCAACATGCCACAG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10538
Forward primer (5'-3') TGCCAGGGATTTTCACAAAGGAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGCACGACCAACATGCCACAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 100	Approximate temperature 53°C

PCR data Exp. ID 10539
Forward primer (5'-3') TGCCAGGGATTTTCACAAAGGAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGCACGACCAACATGCCACAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 100	Approximate temperature 53°C

PCR data Exp. ID 10540
Forward primer (5'-3') TGCCAGGGATTTTCACAAAGGAAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGATGCACGACCAACATGCCACAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 100	Approximate temperature 53°C

PCR data Exp. ID 12240
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1100	Approximate temperature 55°C

PCR data Exp. ID 12241
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1150	Approximate temperature 55°C

PCR data Exp. ID 12242
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 1000	Approximate temperature 55°C

PCR data Exp. ID 12243
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 850	Approximate temperature 55°C

PCR data Exp. ID 12244
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 900	Approximate temperature 55°C

PCR data Exp. ID 12245
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 900	Approximate temperature 55°C

PCR data Exp. ID 12246
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1000	Approximate temperature 55°C

PCR data Exp. ID 12247
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 850	Approximate temperature 55°C

PCR data Exp. ID 12248
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Physalis TA1367: 1200	Approximate temperature 55°C

PCR data Exp. ID 12249
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 1050	Approximate temperature 55°C

PCR data Exp. ID 12250
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 900+1100	Approximate temperature 55°C

PCR data Exp. ID 12251
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Coffea canephora BP409: 1300+1700	Approximate temperature 55°C

PCR data Exp. ID 33332
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 900	Approximate temperature 55°C

PCR data Exp. ID 33333
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 900	Approximate temperature 55°C

PCR data Exp. ID 33334
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1000	Approximate temperature 55°C

PCR data Exp. ID 33335
Forward primer (5'-3') TCGGCCTTGAATGCTATTGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAAGGAAAGGGTAATCTGTGTATCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 850	Approximate temperature 55°C