SGN Marker SSR46

SGN-M719

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 55 High: 50
Repeat motif: AT   Repeat number: 14
Repeat motif:    Repeat number:
Forward primer: CCGAGGCGAATCTTGAATAC
Reverse primer: GCACCATCTCTTGTGCCTCT
Predicted size: 163
Derivations 
This marker was derived from EST read SGN-E92393
Mapped locations  
Map: Tomato - Kazusa and SolCAP markers mapped to genome   
Map version
105 (location: 49372)
Chromosome
11
Position
5.46 MB
Confidence
uncalculated
Protocol
unknown


Tomato - Kazusa and SolCAP markers mapped to genome

Map: Kazusa F2-2000 genetic map   
Map version
103 (location: 47307)
Chromosome
11
Position
60.64 cM
Confidence
uncalculated
Protocol
unknown


Kazusa F2-2000 genetic map

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39249)
Chromosome
11
Position
40.00 cM
Confidence
F(LOD3)
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 140
Forward primer (5'-3')
CCGAGGCGAATCTTGAATAC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
GCACCATCTCTTGTGCCTCT
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 375+375
Solanum pennellii LA716: 375+400
Approximate temperature
50°C
  
Overgo hybridization and physical mapping 
SSR46 was used as an overgo probe on plate 9 [well H10]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
CTCACGAGAAAAGGGAAGAAGAAG
B sequence
ACCATCTCTTGTGCCTCTTCTTCT
AB sequence
CTCACGAGAAAAGGGAAGAAGAAGAGGCAC
Marker sequence
TAAAGATGGGGGAGGCCGAGGCGAATCTTGAATACAACATATGATTACTT
TACCAGAATACTATATATATATATATATATATATATATATATATATGTTA
ACTTGGGGTTGTTGTAGGAATTCCTAAATTTCATCTCTCACGAGAAAAGG
GAAGAAGAAGAGGCACAAGAGATGGTGCTAAAAGTTGATTTGCACTGTGA
AGGTTGTGCTAGAAAAGTTGCAAGAGCATTGAAAGGCCTTCAAGAAATAG
ATGAAGTGACGATCGATTACGAGGAAAGTAAGGTGGTGGTGAAAGGAAAA
AATGTAGATCCACTTAAAGTGTGTGAAAGGGTGGAAAGGAAAAGTGGTAG
AAAAGTTGAACTTATTTCACATATGACAAAGCCATTTGAAGAAAATACAA
AGGAAGAAGAAATCAAGAAAGAAGAAGAGAAAAAAGATGAGCCTCCTCCT
GAAATAACACTTGTGTTGAACGTTCAAATGCATTGTGATGCTTGTGCTCA
AGTTCTTCAGAAGCAAATCCGCAAAATCAAAAGTGTAGAATGTGTTACGA
CAGATATTG
Genomic location of SSR46  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D719 marker 719
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