SGN Marker C2_At2g15320

SGN-M7201
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeSingle311010 (SGN)
130722 (CGN)
---
Capsicum annuumSingleSGN-U201337---
ArabidopsisSingleAt2g15320 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38302)
Chromosome
1
Position
151.00 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39565
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
Accessions and product sizes
Solanum lycopersicum TA209
350
Solanum pennellii TA56
350
Approximate temperature
55°C
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Digested band sizes (using amplicon difference)
Solanum lycopersicum TA209
280
Solanum pennellii TA56
350
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 25616)
Chromosome
2
Position
6.67 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 10564
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 380
Approximate temperature
55°C
  
PCR data   Exp. ID 10565
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 380
Approximate temperature
55°C
  
PCR data   Exp. ID 10566
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 380
Approximate temperature
55°C
  
PCR data   Exp. ID 32232
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 380
Approximate temperature
53°C
  
PCR data   Exp. ID 32233
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 380
Approximate temperature
53°C
  
PCR data   Exp. ID 32234
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 380
Approximate temperature
53°C
  
PCR data   Exp. ID 32235
Forward primer (5'-3')
ACAAGTTGACATGGGGAATAACAGC
Digested band sizes (using ApoI)
Solanum lycopersicum LA925: 270
Solanum pennellii LA716: 380
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AGGTTTCTGTGATTTTAAGCAAAGTTG
Enzymes
All possible enzymes for pennellii and and lycopersicum: ApoI,AvaII,HaeIII
  
Accessions and product sizes
Solanum lycopersicum LA925: 380
Solanum pennellii LA716: 380
Approximate temperature
53°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Amplicon sequence alignment, FASTA
At2g15320F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, plain text

Genomic location of C2_At2g15320  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D7201 marker 7201
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