SGN Marker C2_At2g18050

SGN-M7224
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeSingle301167 (SGN)
124830 (CGN)		---
Capsicum annuumSingleSGN-U196565---
Lycopersicon CombinedMultipleSGN-U216158---
Solanum tuberosumSingleSGN-U246538---
ArabidopsisSingleAt2g18050 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38308)
Chromosome
2
Position
83.10 cM
Confidence
I
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39570
Forward primer (5'-3')
TGCCAAGGCTGTTACTCATCCTCC
Accessions and product sizes
Solanum lycopersicum TA209
550
Solanum pennellii TA56
550
Approximate temperature
55°C
Reverse primer (5'-3')
ATAGTTTGTATGAAGCCTTGATTTTG
Digested band sizes (using amplicon difference)
Solanum lycopersicum TA209
130+150+180
Solanum pennellii TA56
320+140
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 25640)
Chromosome
2
Position
7.85 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 10580
Forward primer (5'-3')
TGCCAAGGCTGTTACTCATCCTCC
 
 
Mg+2 concentration
3mM
Reverse primer (5'-3')
ATAGTTTGTATGAAGCCTTGATTTTG
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 350
 Approximate temperature
55°C
  
PCR data   Exp. ID 10581
Forward primer (5'-3')
TGCCAAGGCTGTTACTCATCCTCC
 
 
Mg+2 concentration
3mM
Reverse primer (5'-3')
ATAGTTTGTATGAAGCCTTGATTTTG
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 350
 Approximate temperature
55°C
  
PCR data   Exp. ID 10582
Forward primer (5'-3')
TGCCAAGGCTGTTACTCATCCTCC
 
 
Mg+2 concentration
3mM
Reverse primer (5'-3')
ATAGTTTGTATGAAGCCTTGATTTTG
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 350
 Approximate temperature
55°C
  
PCR data   Exp. ID 10583
Forward primer (5'-3')
TGCCAAGGCTGTTACTCATCCTCC
Digested band sizes (using AluI)
Solanum pennellii LA716: 350
Solanum lycopersicum LA925: 250
 Mg+2 concentration
3mM
Reverse primer (5'-3')
ATAGTTTGTATGAAGCCTTGATTTTG
Enzymes
All possible enzymes for pennellii and and lycopersicum: AluI
All possible enzymes for lycopersicum and pimpinellifolium: BspDI
   
Accessions and product sizes
Solanum lycopersicum LA925: 350
Solanum pennellii LA716: 350
 Approximate temperature
55°C
  
Unigene blast matches for primers 
SGN-U216157
SGN-U216158
SGN-U270784
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 
Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA
Amplicon sequence alignment, FASTA
At2g18050F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, PDF chromatogram
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, PDF chromatogram
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, PDF chromatogram
Forward amplicon sequence for S. pennellii TA56, plain text
Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA
Amplicon sequence alignment, FASTA
At2g18050F.cds.txt, plain text
Forward amplicon sequence for S. pimpinellifolium LA1589, AB1 chromatogram - [View]
Forward amplicon sequence for S. pimpinellifolium LA1589, PDF chromatogram
Forward amplicon sequence for S. pimpinellifolium LA1589, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, PDF chromatogram
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, PDF chromatogram
Forward amplicon sequence for S. pennellii TA56, plain text
Related Markers  

Related Genotyped Markers: These markers have been genotyped and share a name with this marker
Genomic location of C2_At2g18050  
 %2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D7224 marker 7224
User comments 
Please wait, checking for comments. (If comments do not show up, access them here)