Sol Genomics Network

SGN Marker C2_At2g20860

SGN-M7256
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	307971 (SGN) 128374 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200809	-	-	-
Lycopersicon Combined	Multiple	SGN-U217893	-	-	-
Solanum tuberosum	Single	SGN-U248450	-	-	-
Arabidopsis	Single	At2g20860 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46630)
Chromosome 8
Position 22.00 cM
Confidence I(LOD2)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Tobacco N. acuminata
Map version 89 (location: 46460)
Chromosome 8
Position 6.40 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41373)
Chromosome 7
Position 75.70 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43349

Forward primer (5'-3')

ATTGAAGCCACATATACTCATAGAAGC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCCAGATTTTGCAACTTTCTCTACAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38314)
Chromosome 7
Position 43.30 cM
Confidence I(LOD2)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39575

Forward primer (5'-3')

ATTGAAGCCACATATACTCATAGAAGC

Accessions and product sizes

Solanum pennellii TA56

1650

Solanum lycopersicum TA209

850

Approximate temperature

55°C

Reverse primer (5'-3')

TCCAGATTTTGCAACTTTCTCTACAC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

1650

Solanum lycopersicum TA209

850

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25671)
Chromosome 2
Position 8.99 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10611
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 10612
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 10613
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C

PCR data Exp. ID 12952
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 680	Approximate temperature 55°C

PCR data Exp. ID 12953
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 680	Approximate temperature 55°C

PCR data Exp. ID 12954
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 12955
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1100+680	Approximate temperature 55°C

PCR data Exp. ID 12956
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1150	Approximate temperature 55°C

PCR data Exp. ID 12957
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 12958
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Physalis TA1367: 680	Approximate temperature 55°C

PCR data Exp. ID 12959
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 1150+680	Approximate temperature 55°C

PCR data Exp. ID 12960
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12961
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Coffea canephora BP409: 300	Approximate temperature 55°C

PCR data Exp. ID 32911
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1100	Approximate temperature 55°C

PCR data Exp. ID 32912
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 1100+680	Approximate temperature 55°C

PCR data Exp. ID 32913
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1150	Approximate temperature 55°C

PCR data Exp. ID 32914
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1200	Approximate temperature 55°C

PCR data Exp. ID 10614
Forward primer (5'-3') ATTGAAGCCACATATACTCATAGAAGC	Digested band sizes (using DraI) Solanum lycopersicum LA925: 1350 Solanum pennellii LA716: 750+600	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCAGATTTTGCAACTTTCTCTACAC	Enzymes All possible enzymes for pennellii and and lycopersicum: DraI, HinfI, DpnII All possible enzymes for lycopersicum and pimpinellifolium: DraI, HinfI, DpnII
Accessions and product sizes Solanum pennellii LA716: 1350 Solanum lycopersicum LA925: 1350	Approximate temperature 55°C