Sol Genomics Network

SGN Marker C2_At2g27730

SGN-M7327
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308481 (SGN) 130884 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U196637	-	-	-
Lycopersicon Combined	Single	SGN-U219346	-	-	-
Solanum tuberosum	Single	SGN-U248649	-	-	-
Arabidopsis	Single	At2g27730 on TAIR	-	-	-

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 38337)
Chromosome 11
Position 69.00 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39594

Forward primer (5'-3')

TGGAGAAAGAGAAGCTGGAGAAGC

Accessions and product sizes

Solanum pennellii TA56

750

Solanum lycopersicum TA209

750

Approximate temperature

55°C

Reverse primer (5'-3')

TCCTTTGACATTAGGTACCAACCC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

450

Solanum lycopersicum TA209

400

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25744)
Chromosome 2
Position 11.83 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10705
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 880	Approximate temperature 55°C

PCR data Exp. ID 10706
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 880	Approximate temperature 55°C

PCR data Exp. ID 10707
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 880	Approximate temperature 55°C

PCR data Exp. ID 13007
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 300+900	Approximate temperature 55°C

PCR data Exp. ID 13008
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1000	Approximate temperature 55°C

PCR data Exp. ID 13009
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 900	Approximate temperature 55°C

PCR data Exp. ID 13010
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 900	Approximate temperature 55°C

PCR data Exp. ID 13011
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 900	Approximate temperature 55°C

PCR data Exp. ID 13012
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 1200	Approximate temperature 55°C

PCR data Exp. ID 13013
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 1200	Approximate temperature 55°C

PCR data Exp. ID 32620
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 900	Approximate temperature 55°C

PCR data Exp. ID 32933
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 900	Approximate temperature 55°C

PCR data Exp. ID 32934
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 900	Approximate temperature 55°C

PCR data Exp. ID 32935
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1000	Approximate temperature 55°C

PCR data Exp. ID 32936
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 900	Approximate temperature 55°C

PCR data Exp. ID 32937
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 900	Approximate temperature 55°C

PCR data Exp. ID 10708
Forward primer (5'-3') TGGAGAAAGAGAAGCTGGAGAAGC	Digested band sizes (using HaeIII) Solanum pennellii LA716: 500 Solanum lycopersicum LA925: 650	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCCTTTGACATTAGGTACCAACCC	Enzymes All possible enzymes for pennellii and and lycopersicum: HaeIII, DpnII
Accessions and product sizes Solanum pennellii LA716: 880 Solanum lycopersicum LA925: 880	Approximate temperature 55°C