SGN Marker SSR61

SGN-M734

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 50 High: 45
Repeat motif: GA   Repeat number: 24
Repeat motif:    Repeat number:
Forward primer: ATGCCTTATGGAAACAACGC
Reverse primer: CGGGTGTACGAATGTCTTTG
Predicted size: 237
Derivations 
This marker was derived from EST read SGN-E56863
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 39281)
Chromosome
1
Position
33.00 cM
Confidence
I
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 5
Forward primer (5'-3')
ATGCCTTATGGAAACAACGC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
CGGGTGTACGAATGTCTTTG
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 148+190
Solanum lycopersicum LA925: 190+190
 Approximate temperature
50°C
  
Overgo hybridization and physical mapping 
SSR61 was used as an overgo probe on plate 11 [well A1]

Plausible BAC Matches:   P017M12,   P156M12,   P153H19,   P253J17,   P152B04,   P245J07,   P186O04,   P141O05,   P242I01,   P004I15,   P060A06,   P197A14,   P256M10,   P213G13,   P253K16,   P124P20,   P108N12,   P114N14,   P226H22,   P197J06,   P195C20,   P162L13,   P035J07

 Overgo Sequences

A sequence
ACGCTACTTTTTCATGCTCACATC
B sequence
AGGGAGAAACTCCAGTGATGTGAG
AB sequence
ACGCTACTTTTTCATGCTCACATCACTGGA
Marker sequence
AGACTGTAAATCAATTACTTAGTAGACAAATGCAGATGATTGAGTCAACT
TTCAGGATGATTTTAAGATCCCAATACATGATGTGATGATGAACAGAACC
CTACCCACCCCACCACAAAACAAGATCAAGTTTCTGGCCTATGCCTTATG
GAAACAACGCTACTTTTTCATGCTCACATCACTGGAGTTTCTCCCTCTTT
TATACATTCCTCCATTCTACATCTTCTGATACTTAGAAATGGTGCACAAC
AATTTATGTGACAAACTTAAATTGAGAGAGAGAGAGAGAGAGAGAGAGAG
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGAGAGAGAGCGAGAGTG
TTATTTACAAAGACATTCGTACACCCGTTGTTCAAAAG
Genomic location of SSR61  
 %2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D734 marker 734
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