SGN Marker C2_At2g32600

SGN-M7376
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

SynonymsNone
Locus associationsNone
Orthologs in this COSII group 
SpeciesCopiesSequence IDCDS/Edited sequencePeptide sequencePredicted introns
CoffeeMultiple299657 (SGN)
122988 (CGN)
---
Capsicum annuumSingleSGN-U197760---
Lycopersicon CombinedSingleSGN-U218453---
Solanum tuberosumSingleSGN-U248496---
ArabidopsisSingleAt2g32600 on TAIR---
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 38351)
Chromosome
9
Position
16.50 cM
Confidence
I(LOD2)
Protocol
CAPS


Tomato-EXPEN 2000

PCR data   Exp. ID 39604
Forward primer (5'-3')
TGAAGGGAATTACTTGGCTCACAC
Accessions and product sizes
Solanum pennellii TA56
1100
Solanum lycopersicum TA209
1100
Approximate temperature
55°C
Reverse primer (5'-3')
TGTTTTGTTTCCGGATCAAATTGC
Digested band sizes (using amplicon difference)
Solanum lycopersicum TA209
850
Solanum pennellii TA56
1000
Mg+2 concentration
1.5mM
   Enzymes
  

Map: Arabidopsis COSII   
Map version
44 (location: 25793)
Chromosome
2
Position
13.84 MB
Confidence
uncalculated
Protocol
CAPS


Arabidopsis COSII
Other PCR data 
PCR data   Exp. ID 10753
Forward primer (5'-3')
TGAAGGGAATTACTTGGCTCACAC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TGTTTTGTTTCCGGATCAAATTGC
Enzymes
  
Accessions and product sizes
Solanum pennellii LA716: 1300
Approximate temperature
55°C
  
PCR data   Exp. ID 10754
Forward primer (5'-3')
TGAAGGGAATTACTTGGCTCACAC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TGTTTTGTTTCCGGATCAAATTGC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 1300
Approximate temperature
55°C
  
PCR data   Exp. ID 10755
Forward primer (5'-3')
TGAAGGGAATTACTTGGCTCACAC
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TGTTTTGTTTCCGGATCAAATTGC
Enzymes
  
Accessions and product sizes
Solanum pimpinellifolium LA1589: 1300
Approximate temperature
55°C
  
PCR data   Exp. ID 32254
Forward primer (5'-3')
TGAAGGGAATTACTTGGCTCACAC
Digested band sizes (using HinfI)
Solanum pennellii LA716: 200+350+500
Solanum lycopersicum LA925: 500+550
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
TGTTTTGTTTCCGGATCAAATTGC
Enzymes
All possible enzymes for pennellii and and lycopersicum: BglII,BstYI,HinfI,SspI,PflNI,DpnII,Hpy188I
  
Accessions and product sizes
Solanum lycopersicum LA925: 1300
Solanum pennellii LA716: 1300
Approximate temperature
55°C
  
Unigene blast matches for primers 
Universal primers for Asterid species 
No additional PCR data found.
Other COSII sequence data 

BLASTX result of original unigene sequences against Arabidopsis protein database

Alignment of Arabidopsis CDS and edited Asterid unigenes, FASTA
Alignment of DNA and translated peptides from Arabidopsis CDS and edited Asterid unigenes, plain text
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, ClustalW
Alignment of translated peptides from Arabidopsis CDS and edited Asterid unigenes, FASTA

Input file for PAUP, NEXUS format
Phylogenetic tree
Phylogenetic tree   |   [View]

Amplicon sequence alignment, FASTA
At2g32600F.cds.txt, plain text
Forward amplicon sequence for S. lycopersicum TA209, AB1 chromatogram - [View]
Forward amplicon sequence for S. lycopersicum TA209, plain text
Forward amplicon sequence for S. pennellii TA56, AB1 chromatogram - [View]
Forward amplicon sequence for S. pennellii TA56, plain text
Forward amplicon sequence for S. hirtum 120062 plant #28, AB1 chromatogram - [View]
Forward amplicon sequence for S. hirtum 120062 plant #28, plain text
Forward amplicon sequence for S. quitoense plant #3, AB1 chromatogram - [View]
Forward amplicon sequence for S. quitoense plant #3, plain text

Genomic location of C2_At2g32600  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D7376 marker 7376
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