Sol Genomics Network

SGN Marker C2_At2g32970

SGN-M7381
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	312062 (SGN) 127803 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U223302	-	-	-
Solanum tuberosum	Single	SGN-U253046	-	-	-
Arabidopsis	Single	At2g32970 on TAIR	-	-	-

Mapped locations

Map: Pepper-COSII
Map version 58 (location: 41384)
Chromosome 7
Position 53.90 cM
Confidence I
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43360

Forward primer (5'-3')

TCTGTGGGAAAGTACGTATGCTCC

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TCTGTTGCAGGCTGTGAAAAATGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38354)
Chromosome 7
Position 40.70 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39607

Forward primer (5'-3')

TCTGTGGGAAAGTACGTATGCTCC

Accessions and product sizes

Solanum pennellii TA56

1300

Solanum lycopersicum TA209

1300

Approximate temperature

55°C

Reverse primer (5'-3')

TCTGTTGCAGGCTGTGAAAAATGC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

800

Solanum lycopersicum TA209

750

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25798)
Chromosome 2
Position 14.00 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10760
Forward primer (5'-3') TCTGTGGGAAAGTACGTATGCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTGCAGGCTGTGAAAAATGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 10761
Forward primer (5'-3') TCTGTGGGAAAGTACGTATGCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTGCAGGCTGTGAAAAATGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 10762
Forward primer (5'-3') TCTGTGGGAAAGTACGTATGCTCC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTGCAGGCTGTGAAAAATGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 10763
Forward primer (5'-3') TCTGTGGGAAAGTACGTATGCTCC	Digested band sizes (using AluI) Solanum lycopersicum LA925: 220 Solanum pennellii LA716: 320	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTGTTGCAGGCTGTGAAAAATGC	Enzymes All possible enzymes for pennellii and and lycopersicum: AluI All possible enzymes for lycopersicum and pimpinellifolium: NlaIII/FatI/CviAII/Hin1II/Hsp92II
Accessions and product sizes Solanum pennellii LA716: 350 Solanum lycopersicum LA925: 350	Approximate temperature 55°C