SGN Marker SSR69

SGN-M742

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 50 High: 42.857
Repeat motif: TAT   Repeat number: 7
Repeat motif:    Repeat number:
Forward primer: TTGGCTGGATTATTCCTGTTG
Reverse primer: GCATTTGATAGAAGGCCAGC
Predicted size: 127
Derivations 
This marker was derived from EST read SGN-E48485
Mapped locations  
Map: Tomato-EXPEN 2000   
Map version
52 (location: 39291)
Chromosome
9
Position
37.00 cM
Confidence
I
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 112
Forward primer (5'-3')
TTGGCTGGATTATTCCTGTTG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
GCATTTGATAGAAGGCCAGC
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 127+127
Approximate temperature
55°C
  
Overgo hybridization and physical mapping 
SSR69 was used as an overgo probe on plate 16 [well C4]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
AATCCAGAACCAGCTGGCCTTCTA
B sequence
GTGTTAGGGCATTTGATAGAAGGC
AB sequence
AATCCAGAACCAGCTGGCCTTCTATCAAAT
Marker sequence
ATTTAAGATTAGAGTACATACTCCAATTATTATTATTTCCAATAAATTAA
AGTATATATATCCAACAATTTATGAATAGATTTATTGAAACCTAGCTCTC
TAAATTATTAGAAAAGAGTTCAATTTAATTTCTAAACTTTTGAGATCTAA
GAATTGATGGATATATTTAGAGTTATTTAACCCTAATAGATAATTCATCA
AGTCATTATACATATAATATCATAATTAATCCTATCTAAATAACATTAAT
TTTTTAAATAAAATTCCAGTCCTTAAAATCCCATAATGGGGTTTCAGATA
AATCATTGTAATTTTCCATTGGCTGGATTATTCCTGTTGATGATGAACTT
GGTCCAATATTATTATTATTATTATTATTATTATTAATTAGTGGTTGATG
AGGAGGTAATAATAATCCAGAACCAGCTGGCCTTCTATCAAATGCCCTAA
CACATTTTGATTTCTTGTAAACTCTTCACAAGCTGAATTCATGTTGTAAC
TTTAAATTTGTGATATGATT
Genomic location of SSR69  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D742 marker 742
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