Sol Genomics Network

SGN-M7423
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302716 (SGN) 120204 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U198927	-	-	-
Lycopersicon Combined	Single	SGN-U216530	-	-	-
Solanum tuberosum	Single	SGN-U247062	-	-	-
Arabidopsis	Single	At2g36930 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39617

Forward primer (5'-3')

TCACAGGAGCGTGAAGAAGCG

Accessions and product sizes

Solanum lycopersicum TA209

350

Solanum pennellii TA56

350

Approximate temperature

55°C

Reverse primer (5'-3')

TCTTCGTCTTGAAATGCTCATCCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10800
Forward primer (5'-3') TCACAGGAGCGTGAAGAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTCGTCTTGAAATGCTCATCCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 350	Approximate temperature 55°C

PCR data Exp. ID 10801
Forward primer (5'-3') TCACAGGAGCGTGAAGAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTCGTCTTGAAATGCTCATCCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 350	Approximate temperature 55°C

PCR data Exp. ID 10802
Forward primer (5'-3') TCACAGGAGCGTGAAGAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTCGTCTTGAAATGCTCATCCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 350	Approximate temperature 55°C

PCR data Exp. ID 32275
Forward primer (5'-3') TCACAGGAGCGTGAAGAAGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTCGTCTTGAAATGCTCATCCC	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI All possible enzymes for lycopersicum and pimpinellifolium: SsPI,TaqI
Accessions and product sizes Solanum lycopersicum LA925: 350 Solanum pennellii LA716: 350	Approximate temperature 55°C