Sol Genomics Network

SGN Marker C2_At2g43360

SGN-M7503
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	306637 (SGN) 131701 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U201146	-	-	-
Lycopersicon Combined	Single	SGN-U217712	-	-	-
Solanum tuberosum	Single	SGN-U249276	-	-	-
Arabidopsis	Single	At2g43360 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. acuminata
Map version 89 (location: 46474)
Chromosome 6
Position 58.10 cM
Confidence F(LOD3)
Protocol PCR

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41393)
Chromosome 6
Position 110.30 cM
Confidence F(LOD3)
Protocol PCR

Pepper-COSII

PCR data Exp. ID 43369

Forward primer (5'-3')

TCGATCTCCTCTTTCATGGCG

Accessions and product sizes

Approximate temperature

58°C

Reverse primer (5'-3')

TTGAGGACAATACGAACAATCTTC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38389)
Chromosome 6
Position 67.00 cM
Confidence CF(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39638

Forward primer (5'-3')

TCGATCTCCTCTTTCATGGCG

Accessions and product sizes

Solanum pennellii TA56

350

Solanum lycopersicum TA209

350

Approximate temperature

55°C

Reverse primer (5'-3')

TTGAGGACAATACGAACAATCTTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

350

Solanum pennellii TA56

100+200

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25927)
Chromosome 2
Position 18.02 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10856
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 10857
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1300	Approximate temperature 55°C

PCR data Exp. ID 10858
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1300	Approximate temperature 55°C

PCR data Exp. ID 13099
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1250	Approximate temperature 55°C

PCR data Exp. ID 13100
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Physalis TA1367: 900	Approximate temperature 55°C

PCR data Exp. ID 32625
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1250	Approximate temperature 55°C

PCR data Exp. ID 32973
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1250	Approximate temperature 55°C

PCR data Exp. ID 32974
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1250	Approximate temperature 55°C

PCR data Exp. ID 10859
Forward primer (5'-3') TCGATCTCCTCTTTCATGGCG	Digested band sizes (using amplicon difference) Solanum pennellii LA716: 1350 Solanum lycopersicum LA925: 1300	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGGACAATACGAACAATCTTC	Enzymes All possible enzymes for pennellii and and lycopersicum: amplicon difference
Accessions and product sizes Solanum pennellii LA716: 1350 Solanum lycopersicum LA925: 1300	Approximate temperature 55°C