Sol Genomics Network

SGN-M7549
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300403 (SGN) 120672 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U214743	-	-	-
Solanum tuberosum	Single	SGN-U258164	-	-	-
Arabidopsis	Single	At2g46820 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39648

Forward primer (5'-3')

TGGCATCGTCGCAATGTGTGAC

Accessions and product sizes

Solanum pennellii TA56

750

Solanum lycopersicum TA209

750

Approximate temperature

58°C

Reverse primer (5'-3')

AAGCTCTGATGGTAGCTCAGTGG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

750

Solanum pennellii TA56

650

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10892
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 750	Approximate temperature 55°C

PCR data Exp. ID 10893
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 750	Approximate temperature 55°C

PCR data Exp. ID 10894
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 750	Approximate temperature 55°C

PCR data Exp. ID 32627
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 800	Approximate temperature 55°C

PCR data Exp. ID 32979
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 800	Approximate temperature 55°C

PCR data Exp. ID 32980
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 800	Approximate temperature 55°C

PCR data Exp. ID 10895
Forward primer (5'-3') TGGCATCGTCGCAATGTGTGAC	Digested band sizes (using HinfI) Solanum lycopersicum LA925: 650 Solanum pennellii LA716: 750	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AAGCTCTGATGGTAGCTCAGTGG	Enzymes All possible enzymes for pennellii and and lycopersicum: HinfI All possible enzymes for lycopersicum and pimpinellifolium: HinfI
Accessions and product sizes Solanum lycopersicum LA925: 750 Solanum pennellii LA716: 750	Approximate temperature 55°C