Sol Genomics Network

SGN-M7713
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	311444 (SGN) 127732 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U217431	-	-	-
Arabidopsis	Single	At3g12290 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39694

Forward primer (5'-3')

TCGGCAAGCTTGCAATGAAAGG

Accessions and product sizes

Solanum lycopersicum TA209

180

Solanum pennellii TA56

180

Approximate temperature

55°C

Reverse primer (5'-3')

TTGCTTCGACCAACCACAACTGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11027
Forward primer (5'-3') TCGGCAAGCTTGCAATGAAAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCTTCGACCAACCACAACTGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 280	Approximate temperature 55°C

PCR data Exp. ID 11028
Forward primer (5'-3') TCGGCAAGCTTGCAATGAAAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCTTCGACCAACCACAACTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 280	Approximate temperature 55°C

PCR data Exp. ID 11029
Forward primer (5'-3') TCGGCAAGCTTGCAATGAAAGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCTTCGACCAACCACAACTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 280	Approximate temperature 55°C

PCR data Exp. ID 11030
Forward primer (5'-3') TCGGCAAGCTTGCAATGAAAGG	Digested band sizes (using HinfI) Solanum pennellii LA716: 200 Solanum lycopersicum LA925: 280	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGCTTCGACCAACCACAACTGC	Enzymes All possible enzymes for pennellii and and lycopersicum: ApoI,HinfI,TaqI,Tsp509I All possible enzymes for lycopersicum and pimpinellifolium: ApoI,Tsp509I
Accessions and product sizes Solanum pennellii LA716: 280 Solanum lycopersicum LA925: 280	Approximate temperature 55°C