Sol Genomics Network

SGN-M7746
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	301494 (SGN) 124467 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200183	-	-	-
Lycopersicon Combined	Single	SGN-U215907	-	-	-
Solanum tuberosum	Multiple	SGN-U245219	-	-	-
Arabidopsis	Single	At3g15190 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41388

Forward primer (5'-3')

ATTTACCACAAGGCTAAAAAATCTG

Accessions and product sizes

Solanum lycopersicum TA209

2300

Solanum pennellii TA56

2300

Approximate temperature

53°C

Reverse primer (5'-3')

TCAATGTCTCCAATGCTTCCAAAAC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

720

Solanum pennellii TA56

750

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 11052
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 2300	Approximate temperature 53°C

PCR data Exp. ID 11053
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 2300	Approximate temperature 53°C

PCR data Exp. ID 11054
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2300	Approximate temperature 53°C

PCR data Exp. ID 13211
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 2300	Approximate temperature 55°C

PCR data Exp. ID 13212
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 2300	Approximate temperature 55°C

PCR data Exp. ID 13213
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 2500	Approximate temperature 55°C

PCR data Exp. ID 13214
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 2500	Approximate temperature 55°C

PCR data Exp. ID 13215
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Physalis TA1367: 1800	Approximate temperature 55°C

PCR data Exp. ID 32633
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 2300	Approximate temperature 55°C

PCR data Exp. ID 33021
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 2300	Approximate temperature 55°C

PCR data Exp. ID 33022
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 2500	Approximate temperature 55°C

PCR data Exp. ID 33023
Forward primer (5'-3') ATTTACCACAAGGCTAAAAAATCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCAATGTCTCCAATGCTTCCAAAAC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 2500	Approximate temperature 55°C