Sol Genomics Network

SGN-M8010
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	310638 (SGN) 125941 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U198946	-	-	-
Lycopersicon Combined	Multiple	SGN-U217811	-	-	-
Solanum tuberosum	Single	SGN-U267247	-	-	-
Arabidopsis	Single	At3g53180 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41490

Forward primer (5'-3')

AGTGGAGCATACCTCTGCTGGGG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

TGAATAATGAATCTTTCTCTAAAGCTTC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 11233
Forward primer (5'-3') AGTGGAGCATACCTCTGCTGGGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATAATGAATCTTTCTCTAAAGCTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1100	Approximate temperature 55°C

PCR data Exp. ID 11234
Forward primer (5'-3') AGTGGAGCATACCTCTGCTGGGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATAATGAATCTTTCTCTAAAGCTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1100	Approximate temperature 55°C

PCR data Exp. ID 11235
Forward primer (5'-3') AGTGGAGCATACCTCTGCTGGGG		Mg⁺² concentration 3mM
Reverse primer (5'-3') TGAATAATGAATCTTTCTCTAAAGCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1150	Approximate temperature 55°C

PCR data Exp. ID 32414
Forward primer (5'-3') AGTGGAGCATACCTCTGCTGGGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGAATAATGAATCTTTCTCTAAAGCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1150	Approximate temperature 55°C