SGN Marker SSR135

SGN-M808

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 45 High: 50
Repeat motif: ATT   Repeat number: 6
Repeat motif:    Repeat number:
Forward primer: TGATCGCTTGTGTCCACCTA
Reverse primer: AAAGGAAGTGATGGAAAGCG
Predicted size: 133
Derivations 
This marker was derived from EST read SGN-E30673
Mapped locations  
Map: Tomato - Kazusa and SolCAP markers mapped to genome   
Map version
105 (location: 49362)
Chromosome
1
Position
76.31 MB
Confidence
uncalculated
Protocol
unknown


Tomato - Kazusa and SolCAP markers mapped to genome

Map: Kazusa F2-2000 genetic map   
Map version
103 (location: 47297)
Chromosome
1
Position
59.46 cM
Confidence
uncalculated
Protocol
unknown


Kazusa F2-2000 genetic map

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39174)
Chromosome
1
Position
54.00 cM
Confidence
F(LOD3)
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 9
Forward primer (5'-3')
TGATCGCTTGTGTCCACCTA
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
AAAGGAAGTGATGGAAAGCG
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 133+133
Solanum pennellii LA716: 133+128
Approximate temperature
50°C
  
Overgo hybridization and physical mapping 
SSR135 was used as an overgo probe on plate 8 [well H5]

Plausible BAC Matches:   None

 Overgo Sequences

A sequence
GAGGAGGAAGAACAGTTACACTTG
B sequence
GGTGCTGGTGTTTCAACAAGTGTA
AB sequence
GAGGAGGAAGAACAGTTACACTTGTTGAAA
Marker sequence
CAAACTAAAGAAAGTGATTTTTAATTGTCTCCTCTATCAGTAAAATCAGC
TGTTACATTCAACATTAACAGAACAATTTTCAACAGTAACTATGAATTAC
AGTCAAAACTATTGAATGAGTAAAAGCTAATCCAACTTTCCCAAGAAATT
TTTTAACTTAGATCCATAATCCCACTATTAATCCCTTTGCCTAATACCAC
TAAAAATTTAAGGAAGAGCAAACCTTTACCTCTGATTGTTGATGGGGTTG
ACAAATTCAGCATCAGAAACTGCAGAAACTTCAACTTGACTGATAATTTC
ATCATCATCTTCCATTGGTGGAGGAAAATTAAGATCTATTAAACTTGTTC
CACCAGTTCTTGATAATTCTAATTTATCTGGAGCAGGAGGAGGAAGAACA
GTTACACTTGTTGAAACACCAGCACCAATAGTATGTGATCGCTTGTGTCC
ACCTAAAGCTTGACCTGATGGAAAAACTCTGTAACAAACAGGGCATAGAT
GTATTTTCTCTTCAATTACTGCATTATTATTATTATTATTATGAGCCGCG
CTTTCCATCACTTCCTTTGATAGA
Genomic location of SSR135  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D808 marker 808
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