SGN Marker SSR136

SGN-M809

SynonymsNone
Locus associationsNone
SSR info 

Annealing temperatures: Low: 45 High: 50
Repeat motif: CAG   Repeat number: 7
Repeat motif:    Repeat number:
Forward primer: GAAACCGCCTCTTTCACTTG
Reverse primer: CAGCAATGATTCCAGCGATA
Predicted size: 148
Derivations 
This marker was derived from EST read SGN-E30198
Mapped locations  
Map: Tomato - Kazusa and SolCAP markers mapped to genome   
Map version
105 (location: 49363)
Chromosome
11
Position
1.87 MB
Confidence
uncalculated
Protocol
unknown


Tomato - Kazusa and SolCAP markers mapped to genome

Map: Kazusa F2-2000 genetic map   
Map version
103 (location: 47298)
Chromosome
11
Position
25.60 cM
Confidence
uncalculated
Protocol
unknown


Kazusa F2-2000 genetic map

Map: Tomato-EXPEN 2000   
Map version
52 (location: 39175)
Chromosome
11
Position
11.00 cM
Confidence
F(LOD3)
Protocol
SSR


Tomato-EXPEN 2000
Other PCR data 
PCR data   Exp. ID 136
Forward primer (5'-3')
GAAACCGCCTCTTTCACTTG
 
 
Mg+2 concentration
1.5mM
Reverse primer (5'-3')
CAGCAATGATTCCAGCGATA
Enzymes
  
Accessions and product sizes
Solanum lycopersicum LA925: 149+149
Solanum pennellii LA716: 137+149
Approximate temperature
50°C
  
Overgo hybridization and physical mapping 
SSR136 was used as an overgo probe on plate 1 [well G5]

Plausible BAC Matches:   P143O06

 Overgo Sequences

A sequence
CAAGCCCATGCTACAACTGAGCAA
B sequence
CTGCAACCCAAGCAATTTGCTCAG
AB sequence
CAAGCCCATGCTACAACTGAGCAAATTGCT
Marker sequence
CAGAAACCGCCTCTTTCACTTGAACAACAGCAGCAGCAGCAGCAGCAGCT
ATGGGGAAAAGCGATGATTCCATTCAGAGAAGGAAGAATAAGAAGAGTAG
AAAGAAGCAAGATGACAAGGTATCTAATCGTATCGCTGGAATCATTGCTG
CTAAGAAACGTCGACTCTCCGGCAAACGCCGTATGTGTGAGGGAATGTGC
TATAGTTTACCTACACCTGAGGATCCATTCAATGAGAGGCATGGAAAAGT
TGATCCTGTAAAGAAGAAGAAGAAGAAGCAGAACCAGTCGAAAAAGGGCA
AGAACCCTATTAAGAAGACCGGTTTACCACAAAATTCTGACAAATTAAAG
GAAGAGTTCCACATGCATGCTCCATCTAAGTTCTTAATTCTCTGTTTGAA
AAACATTCAGGATGACCTTCAGCAGGATGGAGCCTTCAGTGGTAGAGAGG
AAAAGCCTTTCTTCATCCATACATGGGGAATTGAATTCTGGAAATATTTT
ACCAGTGGCAAAGATATTATTGGTACAAATCAAGCCCATGCTACAACTGA
GCAAATTGCTTGGGTTGCAGCAACAACTGCTGATGCCATATCAAGAAAGG
AGAAAGAAGTCTATCAATTAGCAACCCCTACCTTTTATT
Genomic location of SSR136  
%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D809 marker 809
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