Sol Genomics Network

SGN-M8295
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	306985 (SGN) 127128 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U205193	-	-	-
Arabidopsis	Single	At4g18810 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39835

Forward primer (5'-3')

TTCGATCAAGGCGACAATATCACG

Accessions and product sizes

Solanum lycopersicum TA209

350

Solanum pennellii TA56

350

Approximate temperature

55°C

Reverse primer (5'-3')

AGCAATTTCTTCACTGGATATTTTTCC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11424
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 300	Approximate temperature 55°C

PCR data Exp. ID 11425
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 300	Approximate temperature 55°C

PCR data Exp. ID 11426
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 300	Approximate temperature 55°C

PCR data Exp. ID 13424
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 320	Approximate temperature 55°C

PCR data Exp. ID 13425
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 250	Approximate temperature 55°C

PCR data Exp. ID 13426
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 400	Approximate temperature 55°C

PCR data Exp. ID 13427
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 400	Approximate temperature 55°C

PCR data Exp. ID 13428
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Physalis TA1367: 320	Approximate temperature 55°C

PCR data Exp. ID 13429
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 250	Approximate temperature 55°C

PCR data Exp. ID 13430
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 250	Approximate temperature 55°C

PCR data Exp. ID 13431
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 250	Approximate temperature 55°C

PCR data Exp. ID 13432
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 250	Approximate temperature 55°C

PCR data Exp. ID 13433
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Coffea canephora BP409: 250	Approximate temperature 55°C

PCR data Exp. ID 32642
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 300	Approximate temperature 55°C

PCR data Exp. ID 33098
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 300	Approximate temperature 55°C

PCR data Exp. ID 33099
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 300	Approximate temperature 55°C

PCR data Exp. ID 33100
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 400	Approximate temperature 55°C

PCR data Exp. ID 11427
Forward primer (5'-3') TTCGATCAAGGCGACAATATCACG	Digested band sizes (using DraI) Solanum pennellii LA716: 280 Solanum lycopersicum LA925: 300	Mg⁺² concentration 3mM
Reverse primer (5'-3') AGCAATTTCTTCACTGGATATTTTTCC	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI, RsaI. DraI, KpnI
Accessions and product sizes Solanum pennellii LA716: 300 Solanum lycopersicum LA925: 300	Approximate temperature 55°C